methanococcus_mcc_medium

Overview

External ontology

mediadive.medium:J228 (source link unavailable) — METHANOCOCCUS McC MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=228

Composition

flowchart LR
    CultureMech_002588["methanococcus_mcc_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002588 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002588 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_002588 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002588 --> ing3_Yeast_extract
    ing4_Resazurin["Resazurin"]
    CultureMech_002588 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002588 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002588 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002588 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002588 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002588 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_NH4Cl["NH4Cl"]
    CultureMech_002588 --> ing10_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing10_NH4Cl -.-> CHEBI_31206
    ing11_KCl["KCl"]
    CultureMech_002588 --> ing11_KCl
    CHEBI_32588["CHEBI:32588"]
    ing11_KCl -.-> CHEBI_32588
    ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002588 --> ing12_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002588 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002588 --> ing14_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing14_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing15_Na2SeO3["Na2SeO3"]
    CultureMech_002588 --> ing15_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing15_Na2SeO3 -.-> CHEBI_48843
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002588 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002588 --> ing17_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing18_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002588 --> ing18_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing18_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002588 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002588 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002588 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (22)

Preparation

1. Mix ingredients, except cysteine x HCl and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2-CO2 (80:20). Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20), seal with butyl rubber stoppers, and autoclave. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).
2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
3. Adjust final pH to 7.0.
4. Dissolve 0.2 g of Fe(NH4)2(SO4)2 x 6H2O in 5 ml of distilled water containing 2 drops of concentrated HCl. When the Fe(NH4)2(SO4)2 x 6H2O crystal have dissolved, bring volume up to 100 ml.

Target organisms

No target organisms are recorded.

Provenance