Overview
- External ontology
- mediadive.medium:J235 (source link unavailable) — PYSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=235
Composition
flowchart LR
CultureMech_002595["pyse_medium"]
ing0_Peptone["Peptone"]
CultureMech_002595 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002595 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002595 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_002595 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002595 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002595 --> ing5_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002595 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 8 G_PER_L | ||
| 2 | Yeast extract | 3 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.35 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.65 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.65 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.043232Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J235 |
| 2026-03-13T06:19:14.420941Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002595 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.685717+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYSE MEDIUM" to "pyse_medium" for consistent matching |
| 2026-06-05T07:40:16.886665+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |