Overview
- External ontology
- mediadive.medium:J242 (source link unavailable) — JCM MEDIUM No. 242
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=242
Composition
flowchart LR
CultureMech_002603["jcm_medium_no_242"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002603 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002603 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002603 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_NaCl["NaCl"]
CultureMech_002603 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002603 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002603 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002603 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Yeast_extract["Yeast extract"]
CultureMech_002603 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_002603 --> ing8_Trypticase_peptone
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_002603 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Sodium_formate["Sodium formate"]
CultureMech_002603 --> ing10_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing10_Sodium_formate -.-> CHEBI_62965
ing11_NaHCO3["NaHCO3"]
CultureMech_002603 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002603 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002603 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Resazurin["Resazurin"]
CultureMech_002603 --> ing14_Resazurin
CHEBI_8806["CHEBI:8806"]
ing14_Resazurin -.-> CHEBI_8806
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002603 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002603 --> ing16_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002603 --> ing17_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002603 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002603 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002603 --> ing20_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing20_AlK_SO4_2 -.-> CHEBI_86463
ing21_H3BO3["H3BO3"]
CultureMech_002603 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002603 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_Biotin["Biotin"]
CultureMech_002603 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_002603 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002603 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_002603 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_002603 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_002603 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002603 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_002603 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.294118 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.294118 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.294118 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1.588235 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.588235, 1.0] |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.127451 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.127451, 3.0] |
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10196078 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00196078, 0.1] |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.10784314 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.00784314, 0.1] |
| 8 | Yeast extract | 1.96078 G_PER_L | ||
| 9 | Trypticase peptone | 1.96078 G_PER_L | ||
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2.45098 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Sodium formate
CHO2.Na 68.007 g/mol
|
2.45098 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.90196 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.490196 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.490196 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 15 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 17 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 18 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except NaHCO3, L-Cysteine x HCl x H2O and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.062795Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J242 |
| 2026-03-13T03:09:43.037422Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.478829Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002603 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.749149+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 242" to "jcm_medium_no_242" for consistent matching |
| 2026-06-05T07:41:09.994418+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.247892+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |