Overview
- External ontology
- mediadive.medium:J246 (source link unavailable) — PPES-II AGAR MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=246
Composition
flowchart LR
CultureMech_002607["ppes_ii_agar_medium"]
ing0_Peptone["Peptone"]
CultureMech_002607 --> ing0_Peptone
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_002607 --> ing1_Proteose_peptone_no__3
ing2_Soytone["Soytone"]
CultureMech_002607 --> ing2_Soytone
ing3_Yeast_extract["Yeast extract"]
CultureMech_002607 --> ing3_Yeast_extract
ing4_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_002607 --> ing4_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing4_Fe_III__EDTA -.-> CHEBI_30729
ing5_Agar["Agar"]
CultureMech_002607 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_NaCl["NaCl"]
CultureMech_002607 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_KCl["KCl"]
CultureMech_002607 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002607 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002607 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002607 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 2 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 1 G_PER_L | ||
| 3 | Soytone | 1 G_PER_L | ||
| 4 | Yeast extract | 1 G_PER_L | ||
| 5 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
0.1 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Adjust pH to 7.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:16.079682Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J246 |
| 2026-01-28T19:48:16.528375Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.518490Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002607 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.793034+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPES-II AGAR MEDIUM" to "ppes_ii_agar_medium" for consistent matching |
| 2026-06-05T07:40:16.914444+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |