eg_medium_with_10_nacl

CultureMech:002618 bacterial COMPLEX LIQUID
Original name: EG MEDIUM WITH 10% NaCl

Overview

External ontology
mediadive.medium:J260 (source link unavailable) — EG MEDIUM WITH 10% NaCl
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=260 Composition recovery source: https://togomedium.org/medium/M252 (TOGO snapshot of JCM medium J260).

Composition

flowchart LR
    CultureMech_002618["eg_medium_with_10_nacl"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_002618 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_002618 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_002618 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_L__Cystine["L--Cystine"]
    CultureMech_002618 --> ing3_L__Cystine
    CHEBI_16283["CHEBI:16283"]
    ing3_L__Cystine -.-> CHEBI_16283
    ing4_Glucose["Glucose"]
    CultureMech_002618 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Soluble_starch["Soluble starch"]
    CultureMech_002618 --> ing5_Soluble_starch
    ing6_Agar["Agar"]
    CultureMech_002618 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002618 --> ing7_Yeast_extract__BD_Difco_
    ing8_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_002618 --> ing8_Proteose_peptone_No__3__BD_Difco_
    ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_002618 --> ing9_L__Cysteine_HCl_H2O
    ing10_Lab__Lemco_powder__Oxoid_["Lab--Lemco powder (Oxoid)"]
    CultureMech_002618 --> ing10_Lab__Lemco_powder__Oxoid_

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
950 ML_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Na2HPO4
HO4P.2Na 141.958 g/mol
4 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
4 L--Cystine
C6H12N2O4S2 240.306 g/mol
0.2 G_PER_L CHEBI:16283
L-cystine
 Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
5 Glucose
C6H12O6 180.156 g/mol
1.5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
6 Soluble starch 0.5 G_PER_L Role: Carbon source; Properties: Undefined component
7 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
8 Yeast extract (BD-Difco) 5 G_PER_L Properties: Undefined component
9 Proteose peptone No. 3 (BD-Difco) 10 G_PER_L Properties: Undefined component
10 L--Cysteine・HCl・H2O 0.5 G_PER_L Properties: Organic compound, Simple component, Defined component
11 Lab--Lemco powder (Oxoid) 2.4 G_PER_L Properties: Undefined component

Preparation

  1. Add L--cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6--7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.
  2. Use EG medium (see Medium No. [14]) with 100 g/L (final) NaCl, and omit horse blood.

Solutions

HCl

Dose: 50 ML_PER_L

Role: Solvating media; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.110446Z mediadive-import Imported from MediaDive Source: JCM, ID: J260
2026-02-03T11:26:54.324735 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:14.575435Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002618
2026-03-17T19:45:27.857178+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EG MEDIUM WITH 10% NaCl" to "eg_medium_with_10_nacl" for consistent matching
2026-04-05T17:20:25.540071 mark_unavailable_media MARKED_UNAVAILABLE Source database has no composition data. Category: EMPTY. JCM page returns "Nothing found"
2026-05-16T08:57:29.768489+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(2 entries)
2026-08-06T07:56:36.095952+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_jcm_missing_from_togo.py RECOVERED_JCM_COMPOSITION_FROM_TOGO Recovered JCM J260 from source-equivalent TOGO M252. Preserved final ingredients and stock-solution boundaries; did not flatten referenced media or stocks.
2026-08-26T02:12:54.934441+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 6 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).