Overview
- External ontology
- mediadive.medium:J262 (source link unavailable) — METHANOCULLEUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=262
Composition
flowchart LR
CultureMech_002620["methanoculleus_medium"]
ing0_KCl["KCl"]
CultureMech_002620 --> ing0_KCl
CHEBI_32588["CHEBI:32588"]
ing0_KCl -.-> CHEBI_32588
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002620 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002620 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NH4Cl["NH4Cl"]
CultureMech_002620 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002620 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_002620 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NaCl["NaCl"]
CultureMech_002620 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002620 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_NaHCO3["NaHCO3"]
CultureMech_002620 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_002620 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Yeast_extract["Yeast extract"]
CultureMech_002620 --> ing10_Yeast_extract
ing11_Trypticase_peptone["Trypticase peptone"]
CultureMech_002620 --> ing11_Trypticase_peptone
ing12_Resazurin["Resazurin"]
CultureMech_002620 --> ing12_Resazurin
CHEBI_8806["CHEBI:8806"]
ing12_Resazurin -.-> CHEBI_8806
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002620 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002620 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002620 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2SeO3["Na2SeO3"]
CultureMech_002620 --> ing16_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing16_Na2SeO3 -.-> CHEBI_48843
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002620 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_Biotin["Biotin"]
CultureMech_002620 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_002620 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002620 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_002620 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_002620 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002620 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002620 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_002620 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002620 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Lipoic_acid["Lipoic acid"]
CultureMech_002620 --> ing27_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_Lipoic_acid -.-> CHEBI_16494
ing28_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002620 --> ing28_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing28_Nitrilotriacetic_acid -.-> CHEBI_44557
ing29_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002620 --> ing29_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing29_MnSO4_x_n_H2O -.-> CHEBI_86360
CultureMech_002620 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KCl
Cl.K 74.551 g/mol
|
0.348039 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 265 |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.92157 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 265 |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.382350000000001 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.38235, 3.0] | Copied from JCM Medium 265 |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.245098 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 265 |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.237255 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.137255, 0.1] | Copied from JCM Medium 265 |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.137255 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 265 |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
18.6471 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 17.6471, 1.0] | Copied from JCM Medium 265 |
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00196078 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Copied from referenced JCM Medium 265 |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.90196 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Copied from referenced JCM Medium 265 |
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.980392 G_PER_L |
CHEBI:32954
sodium acetate
|
Copied from referenced JCM Medium 265 |
| 11 | Yeast extract | 1.96078 G_PER_L | Copied from referenced JCM Medium 265 | |
| 12 | Trypticase peptone | 1.96078 G_PER_L | Copied from referenced JCM Medium 265 | |
| 13 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 265 |
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Copied from referenced JCM Medium 265 |
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 265 |
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Copied from referenced JCM Medium 265 |
| 17 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.001 G_PER_L |
CHEBI:48843
disodium selenite
|
Copied from referenced JCM Medium 265 |
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Copied from referenced JCM Medium 265 |
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
Copied from referenced JCM Medium 265 |
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
Copied from referenced JCM Medium 265 |
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Copied from referenced JCM Medium 265 |
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Copied from referenced JCM Medium 265 |
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
Copied from referenced JCM Medium 265 |
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
Copied from referenced JCM Medium 265 |
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Copied from referenced JCM Medium 265 |
| 26 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
Copied from referenced JCM Medium 265 |
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Copied from referenced JCM Medium 265 |
| 28 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Copied from referenced JCM Medium 265 |
| 29 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Copied from referenced JCM Medium 265 |
| 30 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Copied from referenced JCM Medium 265 |
| 31 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 265 |
| 32 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 265 |
| 33 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 265 |
| 34 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Copied from referenced JCM Medium 265 |
| 35 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Copied from referenced JCM Medium 265 |
| 36 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 265 |
| 37 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 265 |
Preparation
- Use Medium No. 265 with 6.0 g NaCl (final).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.112836Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J262 |
| 2026-02-03T11:26:54.328076 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:14.580063Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002620 |
| 2026-03-17T19:45:27.861978+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOCULLEUS MEDIUM" to "methanoculleus_medium" for consistent matching |
| 2026-04-04T18:49:19.327583 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from methanogenium_medium (CultureMech:000295) |
| 2026-05-16T08:58:06.372191+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:31.378596+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.918948+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |