r_agar

CultureMech:002627 bacterial COMPLEX SOLID_AGAR
Original name: R AGAR

Overview

External ontology
mediadive.medium:J26 (source link unavailable) — R AGAR
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM Medium 26 | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=26 The JCM R Agar table lists Bacto peptone, yeast extract, malt extract, casamino acids, beef extract, glycerol, Tween 80, magnesium sulfate heptahydrate, agar, and distilled water, with a final pH of 7.2.

Composition

flowchart LR
    CultureMech_002627["r_agar"]
    ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
    CultureMech_002627 --> ing0_Bacto_peptone__BD_Difco_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002627 --> ing1_Yeast_extract__BD_Difco_
    ing2_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
    CultureMech_002627 --> ing2_Malt_extract__BD_Difco_
    ing3_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_002627 --> ing3_Casamino_acids__BD_Difco_
    ing4_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
    CultureMech_002627 --> ing4_Beef_extract__BD_Difco_
    ing5_Glycerol["Glycerol"]
    CultureMech_002627 --> ing5_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing5_Glycerol -.-> CHEBI_17754
    ing6_Tween_80["Tween 80"]
    CultureMech_002627 --> ing6_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing6_Tween_80 -.-> CHEBI_53426
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002627 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_002627 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Distilled_water["Distilled water"]
    CultureMech_002627 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Bacto peptone (BD-Difco) 10 G_PER_L MICRO:0000178 (source link unavailable)
Bacto peptone
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
2 Yeast extract (BD-Difco) 5 G_PER_L FOODON:03315426
yeast extract
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
3 Malt extract (BD-Difco) 5 G_PER_L FOODON:03301056
malt extract
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
4 Casamino acids (BD-Difco) 5 G_PER_L JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
5 Beef extract (BD-Difco) 2 G_PER_L FOODON:03302088
beef extract
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
6 Glycerol
C3H8O3 92.094 g/mol
2 G_PER_L CHEBI:17754
glycerol
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
7 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.05 G_PER_L CHEBI:53426
polysorbate 80
 JCM Medium 26 lists 50 mg/L Tween 80.
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
9 Agar 15 G_PER_L CHEBI:2509
agar
 JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base.
10 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 JCM Medium 26 brings the R Agar recipe to 1 L with distilled water.

Preparation

  1. Adjust pH to 7.2.
  2. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.606731Z mediadive-import Imported from MediaDive Source: JCM, ID: J26
2026-01-28T19:48:20.721439Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:14.625018Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002627
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.910844+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R AGAR" to "r_agar" for consistent matching
2026-06-05T07:41:43.744566+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.069659+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py RESOLVED_R_AGAR_SALINITY_SCORE20 Rebuilt the R Agar base from the JCM Medium 26 table.
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/r_agar_with_3_nacl.yaml.
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/r_agar_with_5_nacl.yaml.
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal supplemented-variant link for bacterial/r_agar_with_0_5_tween_80.yaml.
2026-09-08T00:00:00-07:00 repair_r_agar_salinity_score20.py LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/r_agar_with_10_nacl.yaml.
2026-09-10T00:00:00-07:00 repair_jcm_117_863_score20.py LINKED_JCM_117_863_SCORE20_CHILD Added or refreshed reciprocal link for bacterial/r_agar_with_catalase.yaml.