gnys_medium

CultureMech:002628 bacterial COMPLEX LIQUID
Original name: GNYS MEDIUM

Overview

External ontology
mediadive.medium:J270 (source link unavailable) — GNYS MEDIUM
Applications
  • Microbial cultivation
pH
5.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=270

Composition

flowchart LR
    CultureMech_002628["gnys_medium"]
    ing0_Sodium_gluconate["Sodium gluconate"]
    CultureMech_002628 --> ing0_Sodium_gluconate
    CHEBI_84997["CHEBI:84997"]
    ing0_Sodium_gluconate -.-> CHEBI_84997
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002628 --> ing1_Yeast_extract
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002628 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002628 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NaCl["NaCl"]
    CultureMech_002628 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002628 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002628 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_002628 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002628 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_002628 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002628 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_002628 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002628 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002628 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002628 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002628 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Sodium gluconate
C6H11O7.Na 218.137 g/mol
2.997 G_PER_L CHEBI:84997
sodium gluconate
2 Yeast extract 0.999001 G_PER_L
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.999001 G_PER_L CHEBI:62946
ammonium sulfate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.999001 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 NaCl
Cl.Na 58.443 g/mol
0.1998 G_PER_L CHEBI:26710
sodium chloride
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1998 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.04995 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.062 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 4.0-4.5.
  2. For preparation of solid medium, add 18.0 g/L agar and adjust pH to 5.0 to avoid hydrolysis of agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.137922Z mediadive-import Imported from MediaDive Source: JCM, ID: J270
2026-03-13T06:19:14.631706Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002628
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.918258+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GNYS MEDIUM" to "gnys_medium" for consistent matching
2026-06-05T07:40:16.954792+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.871952+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.