Overview
- External ontology
- mediadive.medium:J274 (source link unavailable) — MJ/YTCT MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 11.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=274
Composition
flowchart LR
CultureMech_002631["mj_ytct_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002631 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002631 --> ing1_Tryptone
ing2_Sodium_tartrate["Sodium tartrate"]
CultureMech_002631 --> ing2_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing2_Sodium_tartrate -.-> CHEBI_63017
ing3_Trisodium_citrate["Trisodium citrate"]
CultureMech_002631 --> ing3_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate -.-> CHEBI_53258
ing4_NaNO3["NaNO3"]
CultureMech_002631 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_002631 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Ferric_citrate["Ferric citrate"]
CultureMech_002631 --> ing6_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing6_Ferric_citrate -.-> CHEBI_144421
ing7_Na2SO4["Na2SO4"]
CultureMech_002631 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002631 --> ing8_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing9_Na2CO3["Na2CO3"]
CultureMech_002631 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_NaCl["NaCl"]
CultureMech_002631 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_K2HPO4["K2HPO4"]
CultureMech_002631 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002631 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002631 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002631 --> ing14_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
ing15_KCl["KCl"]
CultureMech_002631 --> ing15_KCl
CHEBI_32588["CHEBI:32588"]
ing15_KCl -.-> CHEBI_32588
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002631 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002631 --> ing17_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing18_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002631 --> ing18_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing18_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Tryptone | 2 G_PER_L | ||
| 3 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
1 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
|
| 4 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
1 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.25 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.2 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 9 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
40 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
29.703 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.138614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 15 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 16 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 19 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Preparation
- Adjust pH to 11.0-12.0 with KOH and autoclave under a N2 atmosphere. After autoclaving, replace the gas phase with N2-CO2 (80:20) and pressurize to 200 kPa.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.150673Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J274 |
| 2026-01-28T19:48:40.730011Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.662641Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002631 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.953230+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ/YTCT MEDIUM" to "mj_ytct_medium" for consistent matching |
| 2026-06-05T07:41:35.045696+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.162107+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |