nam_agar

CultureMech:002635 bacterial COMPLEX SOLID_AGAR
Original name: NAM AGAR

Overview

External ontology
mediadive.medium:J278 (source link unavailable) — NAM AGAR
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=278 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_002635["nam_agar"]
    ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_002635 --> ing0_Pancreatic_digest_of_casein
    ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_002635 --> ing1_Peptic_digest_of_soybean_meal
    ing2_Glucose["Glucose"]
    CultureMech_002635 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_002635 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_002635 --> ing4_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Dipotassium_phosphate -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_002635 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Defibrinated_sheep_blood["Defibrinated sheep blood"]
    CultureMech_002635 --> ing6_Defibrinated_sheep_blood

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
2 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
3 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
4 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 Defibrinated sheep blood 50 G_PER_L

Preparation

  1. After preparing the Trypticase soy agar plate with 5%sheep blood, spreading the subculture on the agar surface and place one NAM disk aseptically. Incubate the agar plate under a H2-CO2-N2 (1:1:8) gas mixture. To prepare the NAM disk, impregnate with 20 �l filter-sterilized 1.5% NAM (<I>N</I>-acetylmuramic acid, Sigma) aqueous solution to a sterile paper disk (0.64 cm in diameter). Stored the air-dried disk at room temperature.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.160556Z mediadive-import Imported from MediaDive Source: JCM, ID: J278
2026-03-13T06:19:14.684918Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002635
2026-03-17T19:45:27.978087+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NAM AGAR" to "nam_agar" for consistent matching