Overview
- External ontology
- mediadive.medium:J289 (source link unavailable) — MODIFIED MB
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=289
Composition
flowchart LR
CultureMech_002646["modified_mb"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002646 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_K2HPO4["K2HPO4"]
CultureMech_002646 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_002646 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002646 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_NaCl["NaCl"]
CultureMech_002646 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_002646 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002646 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002646 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Yeast_extract["Yeast extract"]
CultureMech_002646 --> ing8_Yeast_extract
ing9_Tryptone["Tryptone"]
CultureMech_002646 --> ing9_Tryptone
ing10_Starch["Starch"]
CultureMech_002646 --> ing10_Starch
CHEBI_28017["CHEBI:28017"]
ing10_Starch -.-> CHEBI_28017
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002646 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002646 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002646 --> ing13_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002646 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002646 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002646 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_KAl_SO4_2["KAl(SO4)2"]
CultureMech_002646 --> ing17_KAl_SO4_2
CHEBI_86463["CHEBI:86463"]
ing17_KAl_SO4_2 -.-> CHEBI_86463
ing18_H3BO3["H3BO3"]
CultureMech_002646 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002646 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002646 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.99108 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.297324 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.297324 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.49554 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
2.98216 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 1.98216, 1.0] |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.198216 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14955400000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.049554, 0.1] |
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49554 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 | Yeast extract | 0.99108 G_PER_L | ||
| 10 | Tryptone | 1.98216 G_PER_L | ||
| 11 | Starch | 9.9108 G_PER_L |
CHEBI:28017
starch
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
KAl(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1e-05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Preparation
- Adjust pH to 7.5.
- After boiling, cool down under a stream of oxygen-free N2 gas, dispense into glass tube anaerobically and autoclave. Before inoculation, add the sterile stock solution of 20 mM Na2S2O3.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.194095Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J289 |
| 2026-03-13T03:09:29.967085Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:14.759867Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002646 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.060690+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED MB" to "modified_mb" for consistent matching |
| 2026-06-05T07:41:37.080347+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.720337+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |