Overview
- External ontology
- mediadive.medium:J292 (source link unavailable) — LACTOBACILLUS PONTIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=292
Composition
flowchart LR
CultureMech_002650["lactobacillus_pontis_medium"]
ing0_Lactobacilli_MRS_broth["Lactobacilli MRS broth"]
CultureMech_002650 --> ing0_Lactobacilli_MRS_broth
ing1_Maltose["Maltose"]
CultureMech_002650 --> ing1_Maltose
CHEBI_17306["CHEBI:17306"]
ing1_Maltose -.-> CHEBI_17306
ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002650 --> ing2_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing3_Hemin_chloride["Hemin chloride"]
CultureMech_002650 --> ing3_Hemin_chloride
CHEBI_50385["CHEBI:50385"]
ing3_Hemin_chloride -.-> CHEBI_50385
ing4_Triethanolamine["Triethanolamine"]
CultureMech_002650 --> ing4_Triethanolamine
CHEBI_28621["CHEBI:28621"]
ing4_Triethanolamine -.-> CHEBI_28621
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Lactobacilli MRS broth | 55 G_PER_L | ||
| 2 |
Maltose
C12H22O11 342.297 g/mol
|
100 G_PER_L |
CHEBI:17306
maltose
|
|
| 3 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
33.3333 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 4 |
Hemin chloride
C34H32ClFeN4O4 651.952 g/mol
|
1 G_PER_L |
CHEBI:50385
hemin
|
|
| 5 |
Triethanolamine
C6H15NO3 149.19 g/mol
|
4 G_PER_L |
CHEBI:28621
triethanolamine
|
Preparation
- Adjust pH to 6.5.
- After autoclaving, cool to 50C and add aseptically 18 ml Maltose-hemin solution.
- Filter-sterilize.
- After filter-sterilizing, store at 4C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.200597Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J292 |
| 2026-03-13T06:19:14.772353Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002650 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.075033+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS PONTIS MEDIUM" to "lactobacillus_pontis_medium" for consistent matching |
| 2026-06-05T07:41:10.735189+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |