cellulomonas_fermentans_medium

CultureMech:002652 bacterial COMPLEX LIQUID
Original name: CELLULOMONAS FERMENTANS MEDIUM

Overview

External ontology
mediadive.medium:J295 (source link unavailable) — CELLULOMONAS FERMENTANS MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=295

Composition

flowchart LR
    CultureMech_002652["cellulomonas_fermentans_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_002652 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002652 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002652 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002652 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002652 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002652 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002652 --> ing6_Yeast_extract
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002652 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
2.32632 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.57895 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.36842 G_PER_L CHEBI:62946
ammonium sulfate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.105263 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0210526 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.00131579 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 Yeast extract 5.26316 G_PER_L
8 NaHCO3
CHO3.Na 84.006 g/mol
0.842105 G_PER_L CHEBI:32139
sodium hydrogencarbonate

Preparation

  1. Adjust pH to 7.4. Separately sterilize 50 ml of 10% cellobiose solution, and add to the medium aseptically.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.208918Z mediadive-import Imported from MediaDive Source: JCM, ID: J295
2026-03-13T06:19:14.786411Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002652
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.091370+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CELLULOMONAS FERMENTANS MEDIUM" to "cellulomonas_fermentans_medium" for consistent matching
2026-06-05T07:40:17.010648+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.887624+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.