Overview
- External ontology
- mediadive.medium:J300 (source link unavailable) — PETROTOGA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=300
Composition
flowchart LR
CultureMech_002658["petrotoga_medium"]
ing0_NaCl["NaCl"]
CultureMech_002658 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002658 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002658 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002658 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KCl["KCl"]
CultureMech_002658 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_HEPES["HEPES"]
CultureMech_002658 --> ing5_HEPES
CHEBI_46756["CHEBI:46756"]
ing5_HEPES -.-> CHEBI_46756
ing6_Yeast_extract["Yeast extract"]
CultureMech_002658 --> ing6_Yeast_extract
ing7_Bacto_peptone["Bacto peptone"]
CultureMech_002658 --> ing7_Bacto_peptone
ing8_Tryptone["Tryptone"]
CultureMech_002658 --> ing8_Tryptone
ing9_KH2PO4["KH2PO4"]
CultureMech_002658 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_002658 --> ing10_Maltose_monohydrate
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 | HEPES | 2.6 G_PER_L |
CHEBI:46756
HEPES
|
|
| 7 | Yeast extract | 1.5 G_PER_L | ||
| 8 | Bacto peptone | 1.5 G_PER_L | ||
| 9 | Tryptone | 1.5 G_PER_L | ||
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 | Maltose monohydrate | 3.6 G_PER_L |
Preparation
- Adjust pH to 7.5. Fill the gas phase with N2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.221869Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J300 |
| 2026-03-13T06:19:14.822314Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002658 |
| 2026-03-17T06:30:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.131999+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PETROTOGA MEDIUM" to "petrotoga_medium" for consistent matching |
| 2026-06-05T07:40:17.017715+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.890129+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |