haloalkaliphile_medium

CultureMech:002663 bacterial COMPLEX LIQUID
Original name: HALOALKALIPHILE MEDIUM

Overview

External ontology
mediadive.medium:J306 (source link unavailable) — HALOALKALIPHILE MEDIUM
Applications
  • Microbial cultivation
pH
6.5
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=306

Composition

flowchart LR
    CultureMech_002663["haloalkaliphile_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002663 --> ing0_Yeast_extract
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_002663 --> ing1_Casamino_acids
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002663 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002663 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002663 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002663 --> ing5_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing6_KCl["KCl"]
    CultureMech_002663 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaCl["NaCl"]
    CultureMech_002663 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Na2CO3["Na2CO3"]
    CultureMech_002663 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2 G_PER_L
2 Casamino acids 2 G_PER_L
3 Na glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.1 G_PER_L CHEBI:32583
calcium sulfate dihydrate
7 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
8 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
9 Na2CO3
CO3.2Na 105.988 g/mol
8 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0 - 9.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.242435Z mediadive-import Imported from MediaDive Source: JCM, ID: J306
2026-01-28T19:48:15.903365Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:14.862977Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002663
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.176480+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOALKALIPHILE MEDIUM" to "haloalkaliphile_medium" for consistent matching
2026-06-05T07:41:07.332229+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.566421+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:59.377602+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.