haloneutrophile_medium (CultureMech:002664)

Overview

External ontology

mediadive.medium:J307 (source link unavailable) — HALONEUTROPHILE MEDIUM

Applications

Microbial cultivation

pH

7.1

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=307

Composition

flowchart LR
    CultureMech_002664["haloneutrophile_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002664 --> ing0_Yeast_extract
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_002664 --> ing1_Casamino_acids
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002664 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002664 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002664 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002664 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_002664 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaCl["NaCl"]
    CultureMech_002664 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002664 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002664 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	Yeast extract	2 G_PER_L
2	Casamino acids	2 G_PER_L
3	Na glutamate C5H8NO4.Na 169.112 g/mol	1 G_PER_L	CHEBI:64220 monosodium glutamate
4	Trisodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	10 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	1 G_PER_L	CHEBI:86158 calcium chloride dihydrate
7	KCl Cl.K 74.551 g/mol	1 G_PER_L	CHEBI:32588 potassium chloride
8	NaCl Cl.Na 58.443 g/mol	200 G_PER_L	CHEBI:26710 sodium chloride
9	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.36 MG_PER_L	CHEBI:86249 iron dichloride tetrahydrate
10	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 MG_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate

Preparation

Add components to distilled water and bring volume to 1.0 L.
Adjust pH to 7.0 - 7.2.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.244550Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J307
2026-01-28T19:48:11.106029Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:14.868803Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002664
2026-03-17T06:29:48.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.182798+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "HALONEUTROPHILE MEDIUM" to "haloneutrophile_medium" for consistent matching
2026-06-05T07:41:07.986624+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.754725+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).