pyrobaculum_arsenaticum_medium (CultureMech:002666)

Overview

External ontology

mediadive.medium:J309 (source link unavailable) — PYROBACULUM ARSENATICUM MEDIUM

Applications

Microbial cultivation

pH

6.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=309

Composition

flowchart LR
    CultureMech_002666["pyrobaculum_arsenaticum_medium"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_002666 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002666 --> ing1_Yeast_extract
    ing2_Sulfur["Sulfur"]
    CultureMech_002666 --> ing2_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing2_Sulfur -.-> CHEBI_26833
    ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002666 --> ing3_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_x_9_H2O -.-> CHEBI_76209
    ing4_Resazurin["Resazurin"]
    CultureMech_002666 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002666 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_KH2PO4["KH2PO4"]
    CultureMech_002666 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002666 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002666 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002666 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002666 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002666 --> ing11_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002666 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002666 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002666 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002666 --> ing15_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing15_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002666 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244

Ingredients (17)

#	Ingredient	Concentration	Ontology	Notes
1	Bacto peptone	0.5 G_PER_L		Copied from referenced JCM Medium 184
2	Yeast extract	0.2 G_PER_L		Copied from referenced JCM Medium 184
3	Sulfur S 32.067 g/mol	20 G_PER_L	CHEBI:26833 sulfur atom	Copied from referenced JCM Medium 184
4	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Copied from referenced JCM Medium 184
5	Resazurin C12H7NO4 229.191 g/mol	0.001 G_PER_L	CHEBI:8806 Resazurin	Copied from referenced JCM Medium 184
6	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.3 G_PER_L	CHEBI:62946 ammonium sulfate	Copied from referenced JCM Medium 184
7	KH2PO4 H2O4P.K 136.084 g/mol	0.28 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Copied from referenced JCM Medium 184
8	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Copied from referenced JCM Medium 184
9	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.07 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Copied from referenced JCM Medium 184
10	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.002 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	Copied from referenced JCM Medium 184
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0018 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Copied from referenced JCM Medium 184
12	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	0.0045 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	Copied from referenced JCM Medium 184
13	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.00022 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Copied from referenced JCM Medium 184
14	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	5e-05 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Copied from referenced JCM Medium 184
15	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3e-05 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Copied from referenced JCM Medium 184
16	VOSO4 x n H2O (H2O)n.O5SV 181.021 g/mol	3e-05 G_PER_L	CHEBI:87020 vanadyl sulfate hydrate	Copied from referenced JCM Medium 184
17	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	1e-05 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Copied from referenced JCM Medium 184

Preparation

Use Medium No. 184 without sulfur, and supplement with 2.0 g/L (final) Na2S2O3.
Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.251491Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J309
2026-02-03T11:26:54.484206	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:14.885758Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002666
2026-03-17T19:45:28.200777+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "PYROBACULUM ARSENATICUM MEDIUM" to "pyrobaculum_arsenaticum_medium" for consistent matching
2026-04-04T18:49:19.206331	copy_referenced_compositions	RESOLVED_REFERENCE	Resolved reference by copying from pyrobaculum_medium (CultureMech:002545)
2026-05-16T08:58:13.542710+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:43.198857+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.629599+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.