jcm_medium_no_312 (CultureMech:002670)

Overview

External ontology

mediadive.medium:J312 (source link unavailable) — JCM MEDIUM No. 312

Applications

Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=312

Composition

flowchart LR
    CultureMech_002670["jcm_medium_no_312"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_002670 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002670 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_002670 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002670 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KSCN["KSCN"]
    CultureMech_002670 --> ing4_KSCN
    CHEBI_30951["CHEBI:30951"]
    ing4_KSCN -.-> CHEBI_30951
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002670 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_EDTA["EDTA"]
    CultureMech_002670 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002670 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002670 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002670 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002670 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002670 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002670 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002670 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_H3BO3["H3BO3"]
    CultureMech_002670 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118

Ingredients (15)

#	Ingredient	Concentration	Ontology	Notes
1	Na2CO3 CO3.2Na 105.988 g/mol	19.802 G_PER_L	CHEBI:29377 sodium carbonate	Copied from referenced JCM Medium 310
2	NaHCO3 CHO3.Na 84.006 g/mol	9.90099 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Copied from referenced JCM Medium 310
3	NaCl Cl.Na 58.443 g/mol	4.9505 G_PER_L	CHEBI:26710 sodium chloride	Copied from referenced JCM Medium 310
4	K2HPO4 HO4P.2K 174.174 g/mol	0.990099 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Copied from referenced JCM Medium 310
5	KSCN CNS.K 97.183 g/mol	7.5 G_PER_L	CHEBI:30951 potassium thiocyanate	Copied from referenced JCM Medium 310
6	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	1 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Copied from referenced JCM Medium 310
7	EDTA C10H16N2O8 292.244 g/mol	5 G_PER_L	CHEBI:4735 ethylenediaminetetraacetic acid	Copied from referenced JCM Medium 310
8	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	2 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Copied from referenced JCM Medium 310
9	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.1 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Copied from referenced JCM Medium 310
10	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.03 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Copied from referenced JCM Medium 310
11	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.2 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Copied from referenced JCM Medium 310
12	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.02 G_PER_L	CHEBI:34887 nickel dichloride	Copied from referenced JCM Medium 310
13	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.03 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Copied from referenced JCM Medium 310
14	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.01 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Copied from referenced JCM Medium 310
15	H3BO3 H3BO3 61.833 g/mol	0.3 G_PER_L	CHEBI:33118 boric acid	Copied from referenced JCM Medium 310

Preparation

Use the Medium No. 310 without KSCN solution. Aseptically add 20.0 ml of 2 M Na2S2O3 solution (filter-sterilized) and 10.0 ml of 1 M KNO3 solution (filter-sterilized) to the medium (1.0 L).

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.256735Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J312
2026-02-03T11:26:54.493340	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:14.901544Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002670
2026-03-17T19:45:28.217879+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "JCM MEDIUM No. 312" to "jcm_medium_no_312" for consistent matching
2026-04-04T21:48:41.461042	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from jcm_medium_no_310 (CultureMech:002668)
2026-04-04T22:04:35.263612	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from jcm_medium_no_310 (CultureMech:002668)
2026-05-16T08:57:36.342334+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:10.085007+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.283245+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.