jcm_medium_no_313

CultureMech:002671 bacterial COMPLEX LIQUID
Original name: JCM MEDIUM No. 313

Overview

External ontology
mediadive.medium:J313 (source link unavailable) — JCM MEDIUM No. 313
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=313 Composition recovery source: https://togomedium.org/medium/M308 (TOGO snapshot of JCM medium J313).

Composition

flowchart LR
    CultureMech_002671["jcm_medium_no_313"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_002671 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_002671 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002671 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaHCO3["NaHCO3"]
    CultureMech_002671 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002671 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Na2CO3
CO3.2Na 105.988 g/mol
20 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component

Preparation

  1. Filter--sterilize KSCN solution and separately autoclave Trace elements solution and MgCl2・6H2O solution. Aseptically add KSCN solution, Trace elements solution and MgCl2・6H2O solution to the medium.
  2. Use the Medium No. [310] without KSCN solution. Aseptically add 40.0ml of 2 M Na2S2O3 solution (filter--sterilized) and 5.0 ml of 1 M KNO3 solution (filter--sterilized) to the medium (1.0 L).

Solutions

20% MgCl2・6H2O solution

Dose: 1 ML_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

KNO3 solution

Dose: 5 ML_PER_L

Role: Mineral source; Properties: Defined component, Solution, Inorganic compound

Na2S2O3 solution

Dose: 40 ML_PER_L

Properties: Defined component, Inorganic compound, Solution

Trace elements solution (see Medium [M305])

Dose: 1 ML_PER_L

Defined in TOGO medium M305.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.258678Z mediadive-import Imported from MediaDive Source: JCM, ID: J313
2026-02-03T11:26:54.495376 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:14.903946Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002671
2026-03-17T19:45:28.220608+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 313" to "jcm_medium_no_313" for consistent matching
2026-04-05T17:20:25.570540 mark_unavailable_media MARKED_UNAVAILABLE Source database has no composition data. Category: EMPTY. JCM page returns "Nothing found"
2026-05-16T08:57:36.348623+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(2 keys) -> list(2 entries)
2026-08-06T07:56:36.104031+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_jcm_missing_from_togo.py RECOVERED_JCM_COMPOSITION_FROM_TOGO Recovered JCM J313 from source-equivalent TOGO M308. Preserved final ingredients and stock-solution boundaries; did not flatten referenced media or stocks.
2026-08-26T02:13:02.142742+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 5 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).