Overview
- External ontology
- mediadive.medium:J315 (source link unavailable) — JCM MEDIUM No. 315
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=315
Composition
flowchart LR
CultureMech_002673["jcm_medium_no_315"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002673 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002673 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na3_EDTA["Na3-EDTA"]
CultureMech_002673 --> ing2_Na3_EDTA
CHEBI_63125["CHEBI:63125"]
ing2_Na3_EDTA -.-> CHEBI_63125
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002673 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002673 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002673 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_NaCl["NaCl"]
CultureMech_002673 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_NH4Cl["NH4Cl"]
CultureMech_002673 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Glucose["Glucose"]
CultureMech_002673 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Yeast_extract["Yeast extract"]
CultureMech_002673 --> ing9_Yeast_extract
ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002673 --> ing10_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing11_NaHCO3["NaHCO3"]
CultureMech_002673 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002673 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_Biotin["Biotin"]
CultureMech_002673 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_002673 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002673 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_002673 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_Riboflavin["Riboflavin"]
CultureMech_002673 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_002673 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002673 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002673 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002673 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Lipoic_acid["Lipoic acid"]
CultureMech_002673 --> ing22_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_Lipoic_acid -.-> CHEBI_16494
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002673 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002673 --> ing24_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing24_FeCl2_x_4_H2O -.-> CHEBI_86249
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002673 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002673 --> ing26_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
ing27_ZnCl2["ZnCl2"]
CultureMech_002673 --> ing27_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing27_ZnCl2 -.-> CHEBI_49976
ing28_H3BO3["H3BO3"]
CultureMech_002673 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002673 --> ing29_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213
CultureMech_002673 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.764706 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.735294 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na3-EDTA
C10H13N2O8.3Na 358.19 g/mol
|
0.0392157 G_PER_L |
CHEBI:63125
EDTA trisodium salt
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00980392 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.245098 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0494118 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0294118, 0.02] |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.196078 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.490196 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
0.490196 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 | Yeast extract | 0.490196 G_PER_L | ||
| 11 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.21569 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.90196 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.490196 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
4.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.4 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 27 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 28 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.019 G_PER_L |
CHEBI:33118
boric acid
|
|
| 30 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 31 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
Preparation
- Mix ingredients except cysteine x HCl x H2O, NaHCO3 and Trace vitamins, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as a 10% solution and Trace vitamins while gassing with CO2. Separately autoclave L-cysteine x HCl x H2O as a 5% solution under a CO2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O solution.
- Adjust pH to 7.0 with 1 N KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.263628Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J315 |
| 2026-03-13T03:09:22.719627Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:14.921705Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002673 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.240125+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 315" to "jcm_medium_no_315" for consistent matching |
| 2026-06-05T07:41:10.109887+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.291280+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |