Overview
- External ontology
- mediadive.medium:J318 (source link unavailable) — ALKALINE AGAR
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=318
Composition
flowchart LR
CultureMech_002676["alkaline_agar"]
ing0_Glucose["Glucose"]
CultureMech_002676 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_002676 --> ing1_Bacto_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002676 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_002676 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_H2O["MgSO4 x H2O"]
CultureMech_002676 --> ing4_MgSO4_x_H2O
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4_x_H2O -.-> CHEBI_32599
ing5_NaCl["NaCl"]
CultureMech_002676 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Na2CO3["Na2CO3"]
CultureMech_002676 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_Agar["Agar"]
CultureMech_002676 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Bacto peptone | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgSO4 x H2O
Mg.O4S 120.368 g/mol
|
0.2 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
100 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Autoclave NaCl and Na2CO3 solutions separately and add the solutions to the medium aseptically.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.278099Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J318 |
| 2026-03-13T06:19:14.954531Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002676 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.277311+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE AGAR" to "alkaline_agar" for consistent matching |
| 2026-06-05T07:40:47.841166+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.783858+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |