Overview
- External ontology
- mediadive.medium:J321 (source link unavailable) — 2 x YT MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=321
Composition
flowchart LR
CultureMech_002679["2_x_yt_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_002679 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002679 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002679 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 16 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Yeast extract | 10 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Adjust pH to 7.4 with NaOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.290247Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J321 |
| 2026-03-13T06:19:14.981771Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002679 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.308818+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "2 x YT MEDIUM" to "2_x_yt_medium" for consistent matching |
| 2026-06-05T07:40:15.767124+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_extracts.py | GROUNDED_BACTERIAL_EXTRACTS_SCORE10 | Applied exact MediaIngredientMech mappings for Tryptone, Yeast extract. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |