Overview
- External ontology
- mediadive.medium:J325 (source link unavailable) — MJ/ANHOX-NO<sub>3</sub> WITH THIOSULFATE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=325
Composition
flowchart LR
CultureMech_002683["mj_anhox_no_sub_3_sub_with_thiosulfate_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002683 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_Na2SiO3["Na2SiO3"]
CultureMech_002683 --> ing1_Na2SiO3
ing2_NaNO3["NaNO3"]
CultureMech_002683 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002683 --> ing3_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing4_NaHCO3["NaHCO3"]
CultureMech_002683 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Biotin["Biotin"]
CultureMech_002683 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Folic_acid["Folic acid"]
CultureMech_002683 --> ing6_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing6_Folic_acid -.-> CHEBI_27470
ing7_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002683 --> ing7_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing7_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing8_Thiamine_HCl["Thiamine HCl"]
CultureMech_002683 --> ing8_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing8_Thiamine_HCl -.-> CHEBI_49105
ing9_Riboflavin["Riboflavin"]
CultureMech_002683 --> ing9_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing9_Riboflavin -.-> CHEBI_17015
ing10_Nicotinic_acid["Nicotinic acid"]
CultureMech_002683 --> ing10_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing10_Nicotinic_acid -.-> CHEBI_15940
ing11_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002683 --> ing11_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing11_Calcium_pantothenate -.-> CHEBI_31345
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_002683 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002683 --> ing13_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
ing14_Lipoic_acid["Lipoic acid"]
CultureMech_002683 --> ing14_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing14_Lipoic_acid -.-> CHEBI_16494
ing15_NaCl["NaCl"]
CultureMech_002683 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16_K2HPO4["K2HPO4"]
CultureMech_002683 --> ing16_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing16_K2HPO4 -.-> CHEBI_131527
ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002683 --> ing17_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002683 --> ing18_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
ing19_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002683 --> ing19_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing19_MgCl2_x_6_H2O -.-> CHEBI_86345
ing20_KCl["KCl"]
CultureMech_002683 --> ing20_KCl
CHEBI_32588["CHEBI:32588"]
ing20_KCl -.-> CHEBI_32588
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002683 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002683 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing23_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002683 --> ing23_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing23_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.04995 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 | Na2SiO3 | 0.599401 G_PER_L | ||
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
0.849151 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 4 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.47752 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.998 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 7 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 8 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 9 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 10 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 11 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 12 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 13 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 14 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 15 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
29.703 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 17 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 18 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.138614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 19 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 20 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 21 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 24 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Preparation
- Distribute medium into culture vessels (e.g., 20 ml of medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. Adjust pH to 7.5, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.301578Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J325 |
| 2026-01-28T19:48:17.417016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:24.899557Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:15.015858Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002683 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.350507+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ/ANHOX-NO<sub>3</sub> WITH THIOSULFATE MEDIUM" to "mj_anhox_no_sub_3_sub_with_thiosulfate_medium" for consistent matching |
| 2026-06-05T07:41:34.887806+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.109175+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |