Overview
- External ontology
- mediadive.medium:J329 (source link unavailable) — HALOBIUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=329
Composition
flowchart LR
CultureMech_002687["halobius_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_002687 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_002687 --> ing1_Yeast_extract
ing2_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002687 --> ing2_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing2_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing3_KCl["KCl"]
CultureMech_002687 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002687 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_NaCl["NaCl"]
CultureMech_002687 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002687 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_Agar["Agar"]
CultureMech_002687 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 7.5 G_PER_L | ( vitamin assay) | |
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
116 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.023 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 6.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.310581Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J329 |
| 2026-01-28T19:48:16.519628Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:15.033689Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002687 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.371106+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBIUS MEDIUM" to "halobius_medium" for consistent matching |
| 2026-06-05T07:41:07.692479+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.696980+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |