potato_sucrose_agar

CultureMech:002692 bacterial COMPLEX SOLID_AGAR
Original name: POTATO-SUCROSE AGAR

Overview

External ontology
mediadive.medium:J333 (source link unavailable) — POTATO-SUCROSE AGAR
Applications
  • Microbial cultivation
pH
5.6
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=333

Composition

flowchart LR
    CultureMech_002692["potato_sucrose_agar"]
    ing0_Potato["Potato"]
    CultureMech_002692 --> ing0_Potato
    ing1_Sucrose["Sucrose"]
    CultureMech_002692 --> ing1_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing1_Sucrose -.-> CHEBI_17992
    ing2_Agar["Agar"]
    CultureMech_002692 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Potato 200 G_PER_L
2 Sucrose
C12H22O11 342.297 g/mol
20 G_PER_L CHEBI:17992
sucrose
3 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 5.6.
  2. Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1.0 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil until melting. Add sucrose and mix thoroughly. Make up to 1.0 L, adjust pH and autoclave for sterilization.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.318098Z mediadive-import Imported from MediaDive Source: JCM, ID: J333
2026-03-13T06:19:15.049603Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002692
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.389254+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "POTATO-SUCROSE AGAR" to "potato_sucrose_agar" for consistent matching
2026-06-05T07:41:42.392928+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.