Overview
- External ontology
- mediadive.medium:J338 (source link unavailable) — PYROBACULUM CALIDIFONTIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=338
Composition
flowchart LR
CultureMech_002697["pyrobaculum_calidifontis_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_002697 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002697 --> ing1_Yeast_extract
ing2_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002697 --> ing2_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing2_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
|
| 2 | Yeast extract | 1 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
3 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Preparation
- Adjust pH to 7.0 with NaOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.333606Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J338 |
| 2026-03-13T06:19:15.090408Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002697 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.436829+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROBACULUM CALIDIFONTIS MEDIUM" to "pyrobaculum_calidifontis_medium" for consistent matching |
| 2026-06-05T07:40:17.058975+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-13T00:00:00-07:00 | repair_pyrobaculum_score10.py | GROUNDED_PYROBACULUM_SCORE10 | Applied exact MediaIngredientMech mappings: Tryptone to MICRO:0000182 and Yeast extract to FOODON:03315426. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |