hnw_medium

CultureMech:002704 bacterial DEFINED LIQUID
Original name: HNW MEDIUM

Overview

External ontology
mediadive.medium:J344 (source link unavailable) — HNW MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=344

Composition

flowchart LR
    CultureMech_002704["hnw_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002704 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_NaNO3["NaNO3"]
    CultureMech_002704 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002704 --> ing2_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing2_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing3_NaHCO3["NaHCO3"]
    CultureMech_002704 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_NaCl["NaCl"]
    CultureMech_002704 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002704 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002704 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002704 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002704 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_KCl["KCl"]
    CultureMech_002704 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002704 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002704 --> ing11_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing12_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002704 --> ing12_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing12_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing13_Biotin["Biotin"]
    CultureMech_002704 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_002704 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002704 --> ing15_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002704 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_Riboflavin["Riboflavin"]
    CultureMech_002704 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002704 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002704 --> ing19_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_Calcium_pantothenate -.-> CHEBI_31345
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_002704 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002704 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Lipoic_acid["Lipoic acid"]
    CultureMech_002704 --> ing22_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing22_Lipoic_acid -.-> CHEBI_16494

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.244618 G_PER_L CHEBI:31206
ammonium chloride
2 NaNO3
NO3.Na 84.994 g/mol
0.978474 G_PER_L CHEBI:63005
sodium nitrate
3 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
9.78474e-05 G_PER_L CHEBI:63939
sodium tungstate dihydrate
4 NaHCO3
CHO3.Na 84.006 g/mol
12.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 NaCl
Cl.Na 58.443 g/mol
29.703 G_PER_L CHEBI:26710
sodium chloride
6 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.138614 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36634 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13861 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
10 KCl
Cl.K 74.551 g/mol
0.326733 G_PER_L CHEBI:32588
potassium chloride
11 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00049505 G_PER_L CHEBI:34887
nickel dichloride
12 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00049505 G_PER_L CHEBI:131361
disodium selenite pentahydrate
13 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.00990099 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
14 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
15 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
16 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
18 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
20 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
21 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized):
  2. Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
  3. Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.349762Z mediadive-import Imported from MediaDive Source: JCM, ID: J344
2026-01-28T19:48:16.284433Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.133446Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002704
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.486674+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HNW MEDIUM" to "hnw_medium" for consistent matching
2026-06-05T07:40:17.068838+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.905629+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.