Overview
- External ontology
- mediadive.medium:J347 (source link unavailable) — SME MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=347
Composition
flowchart LR
CultureMech_002707["sme_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002707 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002707 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_Yeast_extract["Yeast extract"]
CultureMech_002707 --> ing2_Yeast_extract
ing3_Sulfur["Sulfur"]
CultureMech_002707 --> ing3_Sulfur
CHEBI_26833["CHEBI:26833"]
ing3_Sulfur -.-> CHEBI_26833
ing4_Resazurin["Resazurin"]
CultureMech_002707 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002707 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_NaCl["NaCl"]
CultureMech_002707 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002707 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002707 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_002707 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaBr["NaBr"]
CultureMech_002707 --> ing10_NaBr
CHEBI_63004["CHEBI:63004"]
ing10_NaBr -.-> CHEBI_63004
ing11_H3BO3["H3BO3"]
CultureMech_002707 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002707 --> ing12_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing12_SrCl2_x_6_H2O -.-> CHEBI_36385
ing13_KI["KI"]
CultureMech_002707 --> ing13_KI
CHEBI_8346["CHEBI:8346"]
ing13_KI -.-> CHEBI_8346
ing14_Trisodium_citrate["Trisodium citrate"]
CultureMech_002707 --> ing14_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing14_Trisodium_citrate -.-> CHEBI_53258
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002707 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 | Yeast extract | 0.4 G_PER_L | ||
| 4 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
55.4 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
14 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 11 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.06 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 14 |
KI
I.K 166.002 g/mol
|
0.0001 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 15 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.02 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, and autoclave. Separately autoclave 10% (w/v) yeast extract solution, and 5% Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, add the yeast extract solution, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.357573Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J347 |
| 2026-03-13T06:19:15.150207Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002707 |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.506237+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SME MEDIUM" to "sme_medium" for consistent matching |
| 2026-06-05T07:40:17.083624+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.910904+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |