py4s_agar

CultureMech:002722 bacterial COMPLEX SOLID_AGAR
Original name: PY4S AGAR

Overview

External ontology
mediadive.medium:J363 (source link unavailable) — PY4S AGAR
Applications
  • Microbial cultivation
pH
6.7
Notes
JCM Medium 363 PY4S Agar lists, per liter, 75 ml Salt solution I from JCM Medium 133, 75 ml Salt solution II from JCM Medium 133, 10.0 g Trypticase peptone (BD-BBL), 5.0 g Yeast extract (BD-Difco), 0.25 g each Glucose, Cellobiose, Maltose, and Soluble starch, 1.0 ml 0.1% resazurin-Na, 2.5 ml 8% Na2CO3, 0.3 g L-Cysteine HCl H2O, 15.0 g Bacto agar (BD-Difco), and 850 ml Distilled water. The final pH is 6.7, and the medium is prepared anaerobically and poured into rubber-stopped tubes under oxygen-free N2-CO2 (95:5).

Composition

flowchart LR
    CultureMech_002722["py4s_agar"]
    ing0_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_002722 --> ing0_Trypticase_peptone__BD_BBL_
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002722 --> ing1_Yeast_extract__BD_Difco_
    ing2_Glucose["Glucose"]
    CultureMech_002722 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Cellobiose["Cellobiose"]
    CultureMech_002722 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Maltose["Maltose"]
    CultureMech_002722 --> ing4_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing4_Maltose -.-> CHEBI_17306
    ing5_Soluble_starch["Soluble starch"]
    CultureMech_002722 --> ing5_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing5_Soluble_starch -.-> CHEBI_28017
    ing6_L_Cysteine_HCl_H2O["L-Cysteine HCl H2O"]
    CultureMech_002722 --> ing6_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing7_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_002722 --> ing7_Bacto_agar__BD_Difco_
    CHEBI_2509["CHEBI:2509"]
    ing7_Bacto_agar__BD_Difco_ -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_002722 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_002722 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_002722 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone (BD-BBL) 10.0 G_PER_L JCM Medium 363 lists 10.0 g/L Trypticase peptone (BD-BBL).
2 Yeast extract (BD-Difco) 5.0 G_PER_L JCM Medium 363 lists 5.0 g/L Yeast extract (BD-Difco).
3 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
 JCM Medium 363 lists 0.25 g/L Glucose.
4 Cellobiose
C12H22O11 342.297 g/mol
0.25 G_PER_L CHEBI:17057
cellobiose
 JCM Medium 363 lists 0.25 g/L Cellobiose.
5 Maltose
C12H22O11 342.297 g/mol
0.25 G_PER_L CHEBI:17306
maltose
 JCM Medium 363 lists 0.25 g/L Maltose.
6 Soluble starch 0.25 G_PER_L CHEBI:28017
starch
 JCM Medium 363 lists 0.25 g/L Soluble starch.
7 L-Cysteine HCl H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.3 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 JCM Medium 363 lists 0.3 g/L L-Cysteine HCl H2O.
8 Bacto agar (BD-Difco) 15.0 G_PER_L CHEBI:2509
agar
 JCM Medium 363 lists 15.0 g/L Bacto agar (BD-Difco).
9 Distilled water
H2O 18.015 g/mol
850.0 ML_PER_L CHEBI:15377
water
 JCM Medium 363 lists 850.0 ml/L Distilled water.
10 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 JCM Medium 363 prepares and dispenses the medium under oxygen-free N2-CO2 (95:5).
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 JCM Medium 363 prepares and dispenses the medium under oxygen-free N2-CO2 (95:5).

Preparation

  1. Prepare PY4S Agar with Salt solution I, Salt solution II, Trypticase peptone, Yeast extract, Glucose, Cellobiose, Maltose, Soluble starch, resazurin-Na, Na2CO3, L-Cysteine HCl H2O, Bacto agar, and distilled water.
  2. Adjust pH to 6.7.
  3. Autoclave at 121 C for 15 min unless otherwise stated.
  4. Prepare anaerobically and pour into rubber-stopped tubes under oxygen-free N2-CO2 (95:5).

Solutions

Salt Solution I

Dose: 75.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
K2HPO4 7.8 G_PER_L JCM Medium 133 lists 7.8 g/L K2HPO4.
Distilled water 1.0 L JCM Medium 133 lists 1.0 L Distilled water.
JCM Medium 363 adds 75 ml/L Salt solution I from JCM Medium 133; JCM Medium 133 defines this stock as 0.78 g K2HPO4 in 100 ml Distilled water.

Salt Solution II

Dose: 75.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
KH2PO4 4.7 G_PER_L JCM Medium 133 lists 4.7 g/L KH2PO4.
NaCl 11.8 G_PER_L JCM Medium 133 lists 11.8 g/L NaCl.
(NH4)2SO4 12.0 G_PER_L JCM Medium 133 lists 12.0 g/L (NH4)2SO4.
CaCl2 1.2 G_PER_L JCM Medium 133 lists 1.2 g/L CaCl2.
MgSO4 x H2O 2.5 G_PER_L JCM Medium 133 lists 2.5 g/L MgSO4 x H2O.
Distilled water 1.0 L JCM Medium 133 lists 1.0 L Distilled water.
JCM Medium 363 adds 75 ml/L Salt solution II from JCM Medium 133; JCM Medium 133 defines this stock as KH2PO4, NaCl, (NH4)2SO4, CaCl2, and MgSO4 x H2O in 100 ml Distilled water.

0.1% (w/v) resazurin-Na

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Sodium resazurin 1.0 G_PER_L JCM Medium 363 lists 1.0 g/L Sodium resazurin.
JCM Medium 363 adds 1.0 ml/L 0.1% (w/v) resazurin-Na.

8% (w/v) Na2CO3

Dose: 2.5 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2CO3 80.0 G_PER_L JCM Medium 363 lists 80.0 g/L Na2CO3.
JCM Medium 363 adds 2.5 ml/L 8% (w/v) Na2CO3.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.400680Z mediadive-import Imported from MediaDive Source: JCM, ID: J363
2026-03-09T08:04:22.894758+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:15.258092Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002722
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.630310+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PY4S AGAR" to "py4s_agar" for consistent matching
2026-05-16T09:04:29.685959+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:42.884046+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.532121+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-06T00:00:00-07:00 repair_jcm_reference_wrappers_score40.py LINKED_JCM_REFERENCE_WRAPPER_CHILD Added or refreshed reciprocal parent-child link for bacterial/xylanobacter_medium.yaml.
2026-09-11T00:00:00-07:00 repair_togo_m358_score15.py RESOLVED_JCM_363_FOR_TOGO_M358 JCM Medium 363 PY4S Agar lists, per liter, 75 ml Salt solution I from JCM Medium 133, 75 ml Salt solution II from JCM Medium 133, 10.0 g Trypticase peptone (BD-BBL), 5.0 g Yeast extract (BD-Difco), 0.25 g each Glucose, Cellobiose, Maltose, and Soluble starch, 1.0 ml 0.1% resazurin-Na, 2.5 ml 8% Na2CO3, 0.3 g L-Cysteine HCl H2O, 15.0 g Bacto agar (BD-Difco), and 850 ml Distilled water. The final pH is 6.7, and the medium is prepared anaerobically and poured into rubber-stopped tubes under oxygen-free N2-CO2 (95:5). Corrected MediaDive stock-volume artifacts, expanded Salt solution I and II from JCM Medium 133, moved the resazurin-Na and Na2CO3 stocks to solutions, and restored the source component amounts before linking TOGO M358.
2026-09-11T00:00:00-07:00 repair_togo_m358_score15.py LINKED_TOGO_M358_SUPPLEMENTED_VARIANT Linked TOGO M358 as a plant-residue supplemented variant of PY4S Agar.