lentibacillus_medium

CultureMech:002734 bacterial COMPLEX LIQUID
Original name: LENTIBACILLUS MEDIUM

Overview

External ontology
mediadive.medium:J377 (source link unavailable) — LENTIBACILLUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.2}
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=377 Ingredient amounts use the exact formulation and preparation stage. Separate preparation excerpt: "Add components to distilled water and bring volume to 1.0 L.". Conditional solid option: "For preparation of solid medium, add 20.0 g/L agar.". Agar is included only in M372/M960 in this reviewed variant cohort; no step ordering is invented.

Composition

flowchart LR
    CultureMech_002734["lentibacillus_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002734 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002734 --> ing1_Yeast_extract
    ing2_Na_glutamate["Na glutamate"]
    CultureMech_002734 --> ing2_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing2_Na_glutamate -.-> CHEBI_64220
    ing3_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002734 --> ing3_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Trisodium_citrate -.-> CHEBI_53258
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002734 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KCl["KCl"]
    CultureMech_002734 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_NaCl["NaCl"]
    CultureMech_002734 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002734 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002734 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_distilled_water["distilled water"]
    CultureMech_002734 --> ing9_distilled_water

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Casamino acids 5 G_PER_L 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
2 Yeast extract 5 G_PER_L 5.0 g in the source preparation brought to 1.0 L gives 5 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
3 Na glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
1.0 g in the source preparation brought to 1.0 L gives 1 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
4 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
3.0 g in the source preparation brought to 1.0 L gives 3 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
20.0 g in the source preparation brought to 1.0 L gives 20 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
6 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
2.0 g in the source preparation brought to 1.0 L gives 2 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
7 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
100.0 g in the source preparation brought to 1.0 L gives 100 G_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
36 MG_PER_L CHEBI:86249
iron dichloride tetrahydrate
36.0 mg in the source preparation brought to 1.0 L gives 36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
0.36 mg in the source preparation brought to 1.0 L gives 0.36 MG_PER_L. Separate exact volume excerpt: "Add components to distilled water and bring volume to 1.0 L.".
10 distilled water Water makes the preparation up to 1.0 L. Its separate input volume and mass are unspecified; neither 1 g/L nor a fixed 1 L water dose is supported.

Preparation

  1. Add components to distilled water and bring volume to 1.0 L.
  2. Adjust pH to 7.0 - 7.2.
  3. For preparation of solid medium, add 20.0 g/L agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.445012Z mediadive-import Imported from MediaDive Source: JCM, ID: J377
2026-01-28T19:48:36.904039Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.357221Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002734
2026-03-17T06:30:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.744833+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LENTIBACILLUS MEDIUM" to "lentibacillus_medium" for consistent matching
2026-06-05T07:40:17.149730+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.320947+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-09T14:23:51.976655+00:00 codex CURATED_HALOBACTERIA_VARIANT_CONCENTRATIONS_AND_PREPARATION_STAGES Source-specific ingredient and variant review. Every change has an exact PURL-backed excerpt. Prepared-base quantities, unquantified water/titrant, source limitations, chemical identities and prior history are preserved; no taxa-media assertions are added.