carnobacterium_pleistocaenium_medium

CultureMech:002736 bacterial COMPLEX LIQUID
Original name: CARNOBACTERIUM PLEISTOCAENIUM MEDIUM

Overview

External ontology
mediadive.medium:J379 (source link unavailable) — CARNOBACTERIUM PLEISTOCAENIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=379

Composition

flowchart LR
    CultureMech_002736["carnobacterium_pleistocaenium_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002736 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002736 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_KCl["KCl"]
    CultureMech_002736 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002736 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002736 --> ing4_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002736 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002736 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_D_Glucose["D-Glucose"]
    CultureMech_002736 --> ing7_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing7_D_Glucose -.-> CHEBI_17634
    ing8_Bacto_peptone["Bacto peptone"]
    CultureMech_002736 --> ing8_Bacto_peptone
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_002736 --> ing9_Yeast_extract
    ing10_Resazurin["Resazurin"]
    CultureMech_002736 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002736 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Biotin["Biotin"]
    CultureMech_002736 --> ing12_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_Biotin -.-> CHEBI_15956
    ing13_Folic_acid["Folic acid"]
    CultureMech_002736 --> ing13_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing13_Folic_acid -.-> CHEBI_27470
    ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002736 --> ing14_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002736 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Riboflavin["Riboflavin"]
    CultureMech_002736 --> ing16_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing16_Riboflavin -.-> CHEBI_17015
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002736 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002736 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_002736 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002736 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_Lipoic_acid["Lipoic acid"]
    CultureMech_002736 --> ing21_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing21_Lipoic_acid -.-> CHEBI_16494
    ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002736 --> ing22_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing23_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002736 --> ing23_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing23_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing24_Na2SeO3["Na2SeO3"]
    CultureMech_002736 --> ing24_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing24_Na2SeO3 -.-> CHEBI_48843
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002736 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002736 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002736 --> ing27_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing27_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing28_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002736 --> ing28_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing28_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002736 --> ing29_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
    CultureMech_002736 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
4.9456 G_PER_L CHEBI:26710
sodium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.296736 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 KCl
Cl.K 74.551 g/mol
0.296736 G_PER_L CHEBI:32588
potassium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.098912 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.012364 G_PER_L CHEBI:32583
calcium sulfate dihydrate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.98912 G_PER_L CHEBI:31206
ammonium chloride
7 NaHCO3
CHO3.Na 84.006 g/mol
0.395648 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 D-Glucose
C6H12O6 180.156 g/mol
4.9456 G_PER_L CHEBI:17634
D-glucose
9 Bacto peptone 0.98912 G_PER_L
10 Yeast extract 0.197824 G_PER_L
11 Resazurin
C12H7NO4 229.191 g/mol
0.00098912 G_PER_L CHEBI:8806
Resazurin
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.395648 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
14 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
15 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
18 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
20 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
23 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
24 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
25 Na2SeO3
2Na.O3Se 172.937 g/mol
0.1 G_PER_L CHEBI:48843
disodium selenite
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
28 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.1 G_PER_L CHEBI:86360
manganese(II) sulfate
29 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
30 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
31 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate

Preparation

  1. Mix ingredients, except NaHCO3 and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2 and add NaHCO3 to the medium. Anaerobically dispense the medium into culture vessels, seal with butyl rubber stoppers and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the Na2S x 9H2O solution to the medium. Check final pH to be 7.2-7.4.
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
  3. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.451779Z mediadive-import Imported from MediaDive Source: JCM, ID: J379
2026-01-28T19:48:19.576391Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.382899Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002736
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.773631+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CARNOBACTERIUM PLEISTOCAENIUM MEDIUM" to "carnobacterium_pleistocaenium_medium" for consistent matching
2026-06-05T07:40:52.653153+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.348612+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.