Overview
- External ontology
- mediadive.medium:J382 (source link unavailable) — AGC2 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 1.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=382 Composition recovery source: https://togomedium.org/medium/M377 (TOGO snapshot of JCM medium J382).
Composition
flowchart LR
CultureMech_002739["agc2_medium"]
ing0_yeast_extract["yeast extract"]
CultureMech_002739 --> ing0_yeast_extract
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component |
Preparation
- Adjust pH to 3.0.
- Use Medium No. [92] with 44.2 g/L (final) FeSO4・7H2O, 5 ml 10 N H2SO4 and 0.5 g/L yeast extract. Dissolve ingredients except FeSO4・7H2O, yeast extract and 10 N H2SO4 in 700 ml distilled water and autoclave. Separately dissolve FeSO4・7H2O in 300 ml distilled water with 10 N H2SO4 and autoclave at 110C for 30 min. After cooling, mix the both solutions. Before inoculation, add yeast extract from a sterile stock solution (10%). Checkthe final pH to be 1.5 -- 2.0.
Solutions
Sol. 1
Dose: 700 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1000 ML_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| MgSO4・7H2O | 0.714285714 G_PER_L | Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| K2HPO4 | 0.714285714 G_PER_L | Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| KCl | 0.142857143 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| (NH4)2SO4 | 4.285714286 G_PER_L | Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| Ca(NO3)2 | 14.285714286 MG_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Defined in TOGO medium M377. Local TOGO stock formulation is represented as an inline composition; it is not flattened into final-medium ingredients.
Sol. 2
Dose: 300 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| 10 N H2SO4 | 16.666666667 ML_PER_L | Role: Mineral source; Properties: Defined component, Solution, Inorganic compound |
| Distilled water | 1000 ML_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| FeSO4・7H2O | 44.2 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Defined in TOGO medium M377. Local TOGO stock formulation is represented as an inline composition; it is not flattened into final-medium ingredients.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.458943Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J382 |
| 2026-02-03T11:26:54.748690 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:15.393992Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002739 |
| 2026-03-17T19:45:28.785976+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AGC2 MEDIUM" to "agc2_medium" for consistent matching |
| 2026-04-05T17:20:25.502807 | mark_unavailable_media | MARKED_UNAVAILABLE | Source database has no composition data. Category: EMPTY. JCM page returns "Nothing found" |
| 2026-05-16T08:57:22.433296+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(2 entries) |
| 2026-08-06T07:56:36.081680+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_jcm_missing_from_togo.py | RECOVERED_JCM_COMPOSITION_FROM_TOGO | Recovered JCM J382 from source-equivalent TOGO M377. Preserved final ingredients and stock-solution boundaries; did not flatten referenced media or stocks. |
| 2026-08-26T02:12:46.189514+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 6 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |