middlebrook_7h10_agar_with_oadc_enrichment

CultureMech:002743 bacterial COMPLEX SOLID_AGAR
Original name: MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT

Overview

External ontology
mediadive.medium:J386 (source link unavailable) — MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT
Applications
  • Microbial cultivation
Notes
Source: JCM Medium 386 | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=386 The JCM Middlebrook 7H10 Agar with OADC Enrichment table lists Middlebrook 7H10 agar, glycerol, distilled water, and a 100 ml/L aseptic addition of Middlebrook OADC Enrichment.

Composition

flowchart LR
    CultureMech_002743["middlebrook_7h10_agar_with_oadc_enrichment"]
    ing0_Middlebrook_7H10_agar__BD_Difco_["Middlebrook 7H10 agar (BD-Difco)"]
    CultureMech_002743 --> ing0_Middlebrook_7H10_agar__BD_Difco_
    ing1_Glycerol["Glycerol"]
    CultureMech_002743 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Distilled_water["Distilled water"]
    CultureMech_002743 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Middlebrook_OADC_Enrichment__BD_BBL_["Middlebrook OADC Enrichment (BD-BBL)"]
    CultureMech_002743 --> ing3_Middlebrook_OADC_Enrichment__BD_BBL_

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Middlebrook 7H10 agar (BD-Difco) 19 G_PER_L JCM Medium 386 lists this commercial BD-Difco medium base as supplied.
2 Glycerol
C3H8O3 92.094 g/mol
5 ML_PER_L CHEBI:17754
glycerol
 JCM Medium 386 lists glycerol as 5 ml/L.
3 Distilled water
H2O 18.015 g/mol
895 ML_PER_L CHEBI:15377
water
 JCM Medium 386 uses 895 ml/L distilled water before OADC addition.
4 Middlebrook OADC Enrichment (BD-BBL) 100 ML_PER_L JCM Medium 386 aseptically adds this commercial BD-BBL enrichment.

Preparation

  1. Sterilize the Middlebrook 7H10 agar, glycerol, and distilled water at 121 C for 10 min.
  2. Cool to 50-55 C.
  3. Aseptically add 100 ml/L Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.472084Z mediadive-import Imported from MediaDive Source: JCM, ID: J386
2026-03-13T06:19:15.424569Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002743
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.820479+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT" to "middlebrook_7h10_agar_with_oadc_enrichment" for consistent matching
2026-06-05T07:41:33.901166+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_jcm_middlebrook_hemin_score20.py RESOLVED_JCM_MIDDLEBROOK_HEMIN_SCORE20 Rebuilt the recipe from live JCM Medium 386 and 714 pages.