pygv_agar (CultureMech:002744)

Overview

External ontology

mediadive.medium:J387 (source link unavailable) — PYGV AGAR

Applications

Microbial cultivation

pH

7.5

Notes

JCM Medium 387 PYGV Agar lists, per liter, 0.25 g Peptone, 0.25 g Yeast extract, 20 ml Mineral salt solution from JCM Medium 304, 10 ml 2.5% Glucose solution, 10 ml Vitamin solution from JCM Medium 304, 15 g Agar, and 960 ml Distilled water. The final pH is 7.5. The base medium is mixed, gently heated to a boil, autoclaved, cooled to 45-50 degrees C, supplemented aseptically with filter-sterilized glucose and vitamin solutions, and adjusted to pH 7.5 with sterile KOH if necessary.

Composition

flowchart LR
    CultureMech_002744["pygv_agar"]
    ing0_Peptone["Peptone"]
    CultureMech_002744 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002744 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_002744 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_002744 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_KOH["KOH"]
    CultureMech_002744 --> ing4_KOH
    CHEBI_32035["CHEBI:32035"]
    ing4_KOH -.-> CHEBI_32035

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	0.25 G_PER_L		MediaDive J387 / JCM Medium 387 lists 0.25 g/L Peptone.
2	Yeast extract	0.25 G_PER_L		MediaDive J387 / JCM Medium 387 lists 0.25 g/L Yeast extract.
3	Agar	15.0 G_PER_L	CHEBI:2509 agar	MediaDive J387 / JCM Medium 387 lists 15.0 g/L Agar.
4	Distilled water H2O 18.015 g/mol	960.0 ML_PER_L	CHEBI:15377 water	MediaDive J387 / JCM Medium 387 lists 960.0 ml/L Distilled water.
5	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	JCM Medium 387 adjusts the medium to pH 7.5 with sterile KOH, if necessary.

Preparation

Mix peptone, yeast extract, mineral salt solution, agar, and distilled water.
Gently heat and bring to a boil.
Autoclave at 121 degrees C for 15 min.
Cool to 45-50 degrees C.
Aseptically add the filter-sterilized glucose solution and vitamin solution.
Adjust to pH 7.5 with sterile KOH, if necessary.

Solutions

Mineral salt solution

Dose: 20.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
MgSO4 x 7H2O	29.7 G_PER_L	MediaDive J387 / JCM Medium 304 lists 29.7 g/L MgSO4 x 7H2O.
Nitrilotriacetic acid	10.0 G_PER_L	MediaDive J387 / JCM Medium 304 lists 10.0 g/L Nitrilotriacetic acid.
CaCl2 x 2H2O	3.34 G_PER_L	MediaDive J387 / JCM Medium 304 lists 3.34 g/L CaCl2 x 2H2O.
FeSO4 x 7H2O	99.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 99.0 mg/L FeSO4 x 7H2O.
Na2MoO4 x 2H2O	13.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 13.0 mg/L Na2MoO4 x 2H2O.
Metals 44	50.0 ML_PER_L	JCM Medium 304 adds 50.0 ml/L Metals 44 from JCM Medium 149.
Distilled water	950.0 ML_PER_L	MediaDive J387 / JCM Medium 304 lists 950.0 ml/L Distilled water.

MediaDive J387 / JCM Medium 387 adds 20.0 ml/L Mineral salt solution.

2.5% Glucose solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Glucose	2.5 PERCENT_W_V	MediaDive J387 / JCM Medium 387 lists 2.5 % w/v Glucose.

MediaDive J387 / JCM Medium 387 adds 10.0 ml/L 2.5% Glucose solution.

Vitamin solution

Dose: 10.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Biotin	2.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 2.0 mg/L Biotin.
Folic acid	2.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 2.0 mg/L Folic acid.
Pyridoxine HCl	10.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 10.0 mg/L Pyridoxine HCl.
Riboflavin	5.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 5.0 mg/L Riboflavin.
Thiamine HCl	5.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 5.0 mg/L Thiamine HCl.
Nicotinamide	5.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 5.0 mg/L Nicotinamide.
Calcium pantothenate	5.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 5.0 mg/L Calcium pantothenate.
Vitamin B12	0.1 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 0.1 mg/L Vitamin B12.
p-Aminobenzoic acid	5.0 MG_PER_L	MediaDive J387 / JCM Medium 304 lists 5.0 mg/L p-Aminobenzoic acid.
Distilled water	1.0 L	MediaDive J387 / JCM Medium 304 lists 1.0 L Distilled water.

MediaDive J387 / JCM Medium 387 adds 10.0 ml/L Vitamin solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.473570Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J387
2026-03-13T06:19:15.431194Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002744
2026-03-17T06:30:49.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.827648+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "PYGV AGAR" to "pygv_agar" for consistent matching
2026-06-05T07:40:17.199599+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00	repair_togo_m382_score15.py	RESOLVED_JCM_387_PYGV_AGAR	JCM Medium 387 PYGV Agar lists, per liter, 0.25 g Peptone, 0.25 g Yeast extract, 20 ml Mineral salt solution from JCM Medium 304, 10 ml 2.5% Glucose solution, 10 ml Vitamin solution from JCM Medium 304, 15 g Agar, and 960 ml Distilled water. The final pH is 7.5. The base medium is mixed, gently heated to a boil, autoclaved, cooled to 45-50 degrees C, supplemented aseptically with filter-sterilized glucose and vitamin solutions, and adjusted to pH 7.5 with sterile KOH if necessary.