desulfovibrio_medium

Overview

External ontology

mediadive.medium:J389 (source link unavailable) — DESULFOVIBRIO MEDIUM

Applications

• Microbial cultivation

pH

7.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=389

Composition

flowchart LR
    CultureMech_002746["desulfovibrio_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_002746 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002746 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002746 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002746 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002746 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sodium_lactate["Sodium lactate"]
    CultureMech_002746 --> ing5_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing5_Sodium_lactate -.-> CHEBI_75228
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002746 --> ing6_Yeast_extract
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002746 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002746 --> ing8_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing8_Sodium_thioglycolate -.-> CHEBI_86481
    ing9_Ascorbic_acid["Ascorbic acid"]
    CultureMech_002746 --> ing9_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing9_Ascorbic_acid -.-> CHEBI_22652
    ing10_Resazurin["Resazurin"]
    CultureMech_002746 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806

Ingredients (11)

Preparation

1. Mix ingredients, except FeSO4 x 7H2O, sodium thioglycolate and ascorbic acid, bring to a boil, and cool down to room temperature under a N2 gas stream. Add FeSO4 x 7H2O in 10 ml distilled water, and sodium thioglycolate and ascorbic acid in 10 ml distilled water, and adjust pH to 7.8 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. Adjust pH to 6.8-7.0, if necessary.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Desulfovibrio intestinalis

  Term

  Id

  NCBITaxon:58621

  Label

  Desulfovibrio intestinalis

  Growth metrics

  • Doubling time minutes

    750.0

    Temperature celsius

    37.0

    Evidence

    • Reference

      PMID:10380647

      Supports

      SUPPORT

      Snippet

      Fastest growth with a doubling time of 12.5 h was obtained at 37 degrees C and not at 28 degrees C, the temperature at which the termites were grown.

      Explanation

      Auto-applied from literature query: targeted: Desulfovibrio intestinalis doubling time

    Is max attainment

    True

Provenance