Overview
- External ontology
- mediadive.medium:J392 (source link unavailable) — MARINOBACTER ALKALIPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- JCM Medium 392 Marinobacter alkaliphilus medium lists 37.4 g Marine broth 2216 (BD-Difco), 0.05 g Na2SiO3, 1.0 g Na2CO3, and 1.0 L distilled water. JCM dissolves Marine broth 2216 in 900 ml water, autoclaves that base, separately dissolves Na2SiO3 and Na2CO3 in 100 ml water, autoclaves the carbonate/silicate solution, and adds it to the medium.
Composition
flowchart LR
CultureMech_002748["marinobacter_alkaliphilus_medium"]
ing0_Marine_broth_2216__BD_Difco_["Marine broth 2216 (BD-Difco)"]
CultureMech_002748 --> ing0_Marine_broth_2216__BD_Difco_
ing1_Na2SiO3["Na2SiO3"]
CultureMech_002748 --> ing1_Na2SiO3
CHEBI_60720["CHEBI:60720"]
ing1_Na2SiO3 -.-> CHEBI_60720
ing2_Na2CO3["Na2CO3"]
CultureMech_002748 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_Distilled_water["Distilled water"]
CultureMech_002748 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Marine broth 2216 (BD-Difco) | 37.4 G_PER_L | JCM Medium 392 lists 37.4 g/L Marine broth 2216 (BD-Difco). | |
| 2 |
Na2SiO3
2Na.O3Si 122.063 g/mol
|
0.05 G_PER_L |
CHEBI:60720
sodium silicate
|
JCM Medium 392 lists 0.05 g/L Na2SiO3. |
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.0 G_PER_L |
CHEBI:29377
sodium carbonate
|
JCM Medium 392 lists 1.0 g/L Na2CO3. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 392 lists 1000 ml/L Distilled water. |
Preparation
- Dissolve 37.4 g Marine broth 2216 (BD-Difco) in 900 ml distilled water.
- Autoclave the Marine broth 2216 base.
- Dissolve 0.05 g Na2SiO3 and 1.0 g Na2CO3 in 100 ml distilled water and autoclave.
- Aseptically add the autoclaved Na2SiO3/Na2CO3 solution to the Marine broth 2216 base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.489889Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J392 |
| 2026-02-06T05:10:36.353871Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:15.457417Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002748 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.857972+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARINOBACTER ALKALIPHILUS MEDIUM" to "marinobacter_alkaliphilus_medium" for consistent matching |
| 2026-06-05T07:41:13.159582+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.976060+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-12T00:00:00-07:00 | repair_jcm_j392_marinobacter_score15.py | RESOLVED_JCM_J392_MARINOBACTER_SCORE15 | JCM Medium 392 Marinobacter alkaliphilus medium lists 37.4 g Marine broth 2216 (BD-Difco), 0.05 g Na2SiO3, 1.0 g Na2CO3, and 1.0 L distilled water. JCM dissolves Marine broth 2216 in 900 ml water, autoclaves that base, separately dissolves Na2SiO3 and Na2CO3 in 100 ml water, autoclaves the carbonate/silicate solution, and adds it to the medium. Restored the JCM distilled-water row, grounded Na2SiO3 and Na2CO3, and retained Marine broth 2216 (BD-Difco) as an opaque commercial product. |