halococcus_dombowskii_medium

CultureMech:002758 archaea COMPLEX LIQUID
Original name: HALOCOCCUS DOMBOWSKII MEDIUM

Overview

External ontology
mediadive.medium:J402 (source link unavailable) — HALOCOCCUS DOMBOWSKII MEDIUM
Applications
  • Microbial cultivation
pH
7.4
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=402

Composition

flowchart LR
    CultureMech_002758["halococcus_dombowskii_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002758 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002758 --> ing1_Yeast_extract
    ing2_Tris_base["Tris base"]
    CultureMech_002758 --> ing2_Tris_base
    CHEBI_9754["CHEBI:9754"]
    ing2_Tris_base -.-> CHEBI_9754
    ing3_KCl["KCl"]
    CultureMech_002758 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002758 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002758 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NaCl["NaCl"]
    CultureMech_002758 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Casamino acids 5 G_PER_L
2 Yeast extract 5 G_PER_L
3 Tris base
C4H11NO3 121.136 g/mol
12.1 G_PER_L CHEBI:9754
tris
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
20 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Add components to distilled water and bring volume to 1.0 L.
  2. Adjust pH to 7.4.
  3. For preparation of solid medium, add 20.0 g/L agar.
  4. Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.516696Z mediadive-import Imported from MediaDive Source: JCM, ID: J402
2026-01-28T19:48:13.374796Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.526528Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002758
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.935715+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOCOCCUS DOMBOWSKII MEDIUM" to "halococcus_dombowskii_medium" for consistent matching
2026-06-05T07:41:07.734769+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.703219+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).