acetobacterium_submarinus_medium

Overview

External ontology

mediadive.medium:J403 (source link unavailable) — ACETOBACTERIUM SUBMARINUS MEDIUM

Applications

• Microbial cultivation

pH

7.0

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=403

Composition

flowchart LR
    CultureMech_002759["acetobacterium_submarinus_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_002759 --> ing0_Sea_Salt
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002759 --> ing1_Yeast_extract
    ing2_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002759 --> ing2_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing2_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing3_Na2SeO4["Na2SeO4"]
    CultureMech_002759 --> ing3_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing3_Na2SeO4 -.-> CHEBI_77775
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_002759 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Methylamine["Methylamine"]
    CultureMech_002759 --> ing5_Methylamine
    CHEBI_16830["CHEBI:16830"]
    ing5_Methylamine -.-> CHEBI_16830
    ing6_Sodium_formate["Sodium formate"]
    CultureMech_002759 --> ing6_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing6_Sodium_formate -.-> CHEBI_62965
    ing7_Methanol["Methanol"]
    CultureMech_002759 --> ing7_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing7_Methanol -.-> CHEBI_17790
    ing8_KH2PO4["KH2PO4"]
    CultureMech_002759 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_NH4Cl["NH4Cl"]
    CultureMech_002759 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_Resazurin["Resazurin"]
    CultureMech_002759 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_NaHCO3["NaHCO3"]
    CultureMech_002759 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002759 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002759 --> ing13_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing14_Biotin["Biotin"]
    CultureMech_002759 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002759 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002759 --> ing16_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002759 --> ing17_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing18_Sodium_phosphate_buffer["Sodium phosphate buffer"]
    CultureMech_002759 --> ing18_Sodium_phosphate_buffer
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002759 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_002759 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002759 --> ing21_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002759 --> ing22_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing23_H3BO3["H3BO3"]
    CultureMech_002759 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002759 --> ing24_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002759 --> ing25_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing26_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002759 --> ing26_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing26_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002759 --> ing27_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002759 --> ing28_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (29)

Preparation

1. Mix ingredients except methanol, NaHCO3 and Na2S x 9H2O, bring to a boil and cool down under a stream of N2 gas. Add methanol to the medium and distribute into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers, and autoclave. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Filer-sterilize 8% NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. Before inoculation, add the sterile NaHCO3 and Na2S x 9H2O solutions to the medium, and readjust pH to 7.0, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
2. Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.

Target organisms

No target organisms are recorded.

Provenance