nutrient_agar_with_50_soil_extract_and_5_nacl

CultureMech:002763 bacterial COMPLEX SOLID_AGAR
Original name: NUTRIENT AGAR WITH 50% SOIL EXTRACT AND 5% NaCl

Overview

External ontology
mediadive.medium:J407 (source link unavailable) — NUTRIENT AGAR WITH 50% SOIL EXTRACT AND 5% NaCl
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=407

Composition

flowchart LR
    CultureMech_002763["nutrient_agar_with_50_soil_extract_and_5_nacl"]
    ing0_Peptone["Peptone"]
    CultureMech_002763 --> ing0_Peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_002763 --> ing1_Beef_extract
    ing2_NaCl["NaCl"]
    CultureMech_002763 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_002763 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Peptone 5 G_PER_L
2 Beef extract 3 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride
4 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0.
  2. Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.531095Z mediadive-import Imported from MediaDive Source: JCM, ID: J407
2026-03-13T06:19:15.562548Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002763
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.976418+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NUTRIENT AGAR WITH 50% SOIL EXTRACT AND 5% NaCl" to "nutrient_agar_with_50_soil_extract_and_5_nacl" for consistent matching
2026-06-05T07:41:40.152424+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.