fe_iii_reducer_freshwater_enrichment_medium

Overview

External ontology

mediadive.medium:J412 (source link unavailable) — Fe(III)-REDUCER FRESHWATER ENRICHMENT MEDIUM

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=412

Composition

flowchart LR
    CultureMech_002768["fe_iii_reducer_freshwater_enrichment_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002768 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002768 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_KCl["KCl"]
    CultureMech_002768 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002768 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002768 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002768 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_Na2SeO4["Na2SeO4"]
    CultureMech_002768 --> ing6_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing6_Na2SeO4 -.-> CHEBI_77775
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002768 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002768 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_Biotin["Biotin"]
    CultureMech_002768 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_002768 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002768 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002768 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Riboflavin["Riboflavin"]
    CultureMech_002768 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002768 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002768 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002768 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002768 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Lipoic_acid["Lipoic acid"]
    CultureMech_002768 --> ing18_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18_Lipoic_acid -.-> CHEBI_16494

Ingredients (19)

Preparation

1. Add components except L-cysteine x HCl x H2O and FeCl2 x 4H2O to distilled water, bring volume to 1.0 L and adjust pH to 7.0. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions under a N2 gas atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions. Pressurize the inoculated vessels to 100 kPa H2-CO2 (4:1, v/v) and cultivate without shaking.
2. Comment: For strain JCM 12379, inoculate with a 10% inoculum.
3. Dissolve 108 g FeCl3 in 1.0 L distilled water. Adjust pH to 7.0 with 10 N NaOH. Allow the suspension to sit for 30 min, then readjust pH to 7.0. Centrifuge the suspension at 5,000 x g for 20 min and pour off supernatant. Wash the precipitate with distilled water by resuspending in distilled water followed by the centrifugation. Repeat the washing at least 5 times, then, resuspend in 1.0 L distilled water. Use the Fe(III) suspension as 0.67 M solution. More precisely, check the concentration of Cl- to be less than 1 mM and determine the concentration of Fe(III) by using hydroxylamine as described by Lovley and Phillips (Appl. Environ. Microbiol. 54, 751-757, 1986).
4. Use Trace minerals of Medium No. 151 supplemented with 0.025 g (final) Na2MoO4 x 2H2O, 0.024 g NiCl2 x 6H2O and 0.025 g Na2WO4 x 2H2O (per liter).
5. Comment: For strain JCM 12378, inoculate with a 10% inoculum.

Target organisms

No target organisms are recorded.

Provenance