mjytfsh_medium

CultureMech:002771 bacterial COMPLEX LIQUID
Original name: MJYTFSH MEDIUM

Overview

External ontology
mediadive.medium:J417 (source link unavailable) — MJYTFSH MEDIUM
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=417

Composition

flowchart LR
    CultureMech_002771["mjytfsh_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002771 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002771 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002771 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002771 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002771 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002771 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002771 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_002771 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002771 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002771 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_002771 --> ing10_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing10_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing11_H2WO4["H2WO4"]
    CultureMech_002771 --> ing11_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing11_H2WO4 -.-> CHEBI_36272
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002771 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_Yeast_extract["Yeast extract"]
    CultureMech_002771 --> ing13_Yeast_extract
    ing14_Tryptone["Tryptone"]
    CultureMech_002771 --> ing14_Tryptone
    ing15_Sodium_formate["Sodium formate"]
    CultureMech_002771 --> ing15_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing15_Sodium_formate -.-> CHEBI_62965
    ing16_NaHCO3["NaHCO3"]
    CultureMech_002771 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_Sulfur["Sulfur"]
    CultureMech_002771 --> ing17_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing17_Sulfur -.-> CHEBI_26833
    ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002771 --> ing18_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing18_Na2S_x_9_H2O -.-> CHEBI_76209
    ing19_Biotin["Biotin"]
    CultureMech_002771 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_002771 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002771 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002771 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Riboflavin["Riboflavin"]
    CultureMech_002771 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002771 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002771 --> ing25_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_002771 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002771 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_002771 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.7824 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.890208 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.692384 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.98912 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
1.2364 G_PER_L CHEBI:31206
ammonium chloride
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36301 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13452 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 KCl
Cl.K 74.551 g/mol
0.326409 G_PER_L CHEBI:32588
potassium chloride
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0049456 G_PER_L CHEBI:34887
nickel dichloride
10 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0049456 G_PER_L CHEBI:131361
disodium selenite pentahydrate
11 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
0.0098912 G_PER_L CHEBI:53438
iron(3+) sulfate
12 H2WO4
H2O4W 249.852 g/mol
0.0049456 G_PER_L CHEBI:36272
tungstic acid
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0197824 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 Yeast extract 0.98912 G_PER_L
15 Tryptone 0.98912 G_PER_L
16 Sodium formate
CHO2.Na 68.007 g/mol
0.197824 G_PER_L CHEBI:62965
sodium formate
17 NaHCO3
CHO3.Na 84.006 g/mol
1.97824 G_PER_L CHEBI:32139
sodium hydrogencarbonate
18 Sulfur
S 32.067 g/mol
9.8912 G_PER_L CHEBI:26833
sulfur atom
19 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49456 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
24 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
25 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
26 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
27 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients, except Trace vitamins, NaHCO3 and sulfur, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sterilized sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, sparge for 5 min with the same gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add the sterilized Na2S x 9H2O solution to the medium, and pressurize to 200 kPa H2-CO2 (4:1, v/v). Readjust pH to 5.5, if necessary.
  2. Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.556130Z mediadive-import Imported from MediaDive Source: JCM, ID: J417
2026-03-13T06:19:15.606095Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002771
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.024813+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MJYTFSH MEDIUM" to "mjytfsh_medium" for consistent matching
2026-06-05T07:41:35.154342+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.201477+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.