Overview
- External ontology
- mediadive.medium:J426 (source link unavailable) — BEER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=426 Composition recovery source: https://togomedium.org/medium/M426 (TOGO snapshot of JCM medium J426).
Composition
flowchart LR
CultureMech_002778["beer_medium"]
ing0_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_002778 --> ing0_MgSO4_7H2O
ing1_K2HPO4["K2HPO4"]
CultureMech_002778 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_002778 --> ing2_Sodium_acetate
ing3_Tween_80["Tween 80"]
CultureMech_002778 --> ing3_Tween_80
CHEBI_53426["CHEBI:53426"]
ing3_Tween_80 -.-> CHEBI_53426
ing4_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_002778 --> ing4_MnSO4_xH2O
ing5_Diammonium_citrate["Diammonium citrate"]
CultureMech_002778 --> ing5_Diammonium_citrate
ing6_Glucose["Glucose"]
CultureMech_002778 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_hopped_beer["hopped beer"]
CultureMech_002778 --> ing7_hopped_beer
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_002778 --> ing8_Yeast_extract__BD_Difco_
ing9_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_002778 --> ing9_Beef_extract__BD_Difco_
ing10_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
CultureMech_002778 --> ing10_Casein_peptone__tryptic_digest
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | MgSO4・7H2O | 0.2 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Sodium acetate | 5 G_PER_L | Role: Buffer; Properties: Defined component, Organic compound, Simple component | |
| 4 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 5 | MnSO4・xH2O | 50 MG_PER_L | Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound | |
| 6 | Diammonium citrate | 2 G_PER_L | Role: Nutrient source; Properties: Simple component, Defined component, Organic compound | |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 8 | hopped beer | 1 L | Role: Solvating media; Properties: Undefined component | |
| 9 | Yeast extract (BD-Difco) | 5 G_PER_L |
FOODON:03315426
Yeast extract
|
Properties: Undefined component |
| 10 | Beef extract (BD-Difco) | 10 G_PER_L |
FOODON:03302088
Beef extract
|
Properties: Undefined component |
| 11 | Casein peptone, tryptic digest | 10 G_PER_L |
FOODON:03315719
Casein peptone
|
Properties: Undefined component |
Preparation
- Prepare MRS medium (see Medium No. [1]) in normally hopped beer instead of distilled water. Adjust pH to 5.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.585136Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J426 |
| 2026-02-03T11:26:54.889235 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:15.660063Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002778 |
| 2026-03-17T19:45:29.085925+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BEER MEDIUM" to "beer_medium" for consistent matching |
| 2026-04-05T17:20:25.590670 | mark_unavailable_media | MARKED_UNAVAILABLE | Source database has no composition data. Category: UNKNOWN. Page exists but no clear composition format |
| 2026-05-16T08:57:24.312361+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(2 entries) |
| 2026-08-06T07:56:36.083192+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_jcm_missing_from_togo.py | RECOVERED_JCM_COMPOSITION_FROM_TOGO | Recovered JCM J426 from source-equivalent TOGO M426. Preserved final ingredients and stock-solution boundaries; did not flatten referenced media or stocks. |
| 2026-08-26T02:12:48.602772+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch2.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH2 | Applied local mappings for Beef extract (BD-Difco), Casein peptone, tryptic digest, Yeast extract (BD-Difco). |