Overview
- External ontology
- mediadive.medium:J428 (source link unavailable) — PY MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=428
Composition
flowchart LR
CultureMech_002780["py_medium"]
ing0_Peptone["Peptone"]
CultureMech_002780 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002780 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_002780 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaH2PO4["NaH2PO4"]
CultureMech_002780 --> ing3_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing3_NaH2PO4 -.-> CHEBI_37585
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002780 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
0.05 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.02 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.588529Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J428 |
| 2026-03-13T06:19:15.667375Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002780 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.094436+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY MEDIUM" to "py_medium" for consistent matching |
| 2026-06-05T07:41:42.940987+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.553258+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |