Overview
- External ontology
- mediadive.medium:J442 (source link unavailable) — MODIFIED GROWTH MEDIUM WITH PYRUVATE
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=442
Composition
flowchart LR
CultureMech_002792["modified_growth_medium_with_pyruvate"]
ing0_NaCl["NaCl"]
CultureMech_002792 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002792 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002792 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_002792 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002792 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Peptone["Peptone"]
CultureMech_002792 --> ing5_Peptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_002792 --> ing6_Yeast_extract
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002792 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
184 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
23 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
26.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
5.4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.38 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Peptone | 5 G_PER_L | ||
| 7 | Yeast extract | 1 G_PER_L | ||
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L.
- Adjust pH to 7.5 with 1 M Tris base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.628016Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J442 |
| 2026-03-13T06:19:15.748199Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002792 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.183771+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED GROWTH MEDIUM WITH PYRUVATE" to "modified_growth_medium_with_pyruvate" for consistent matching |
| 2026-06-05T07:41:36.625819+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |