mjtso_medium

CultureMech:002796 bacterial DEFINED LIQUID
Original name: MJTSO MEDIUM

Overview

External ontology
mediadive.medium:J446 (source link unavailable) — MJTSO MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=446

Composition

flowchart LR
    CultureMech_002796["mjtso_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002796 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002796 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002796 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002796 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002796 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002796 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002796 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002796 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Sulfur["Sulfur"]
    CultureMech_002796 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002796 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2_EDTA["Na2-EDTA"]
    CultureMech_002796 --> ing10_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing10_Na2_EDTA -.-> CHEBI_64734
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002796 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002796 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002796 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_ZnCl2["ZnCl2"]
    CultureMech_002796 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_002796 --> ing15_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing15_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing16_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
    CultureMech_002796 --> ing16_Na2WO2_x_2_H2O
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002796 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_002796 --> ing18_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing18_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing19_H2SeO3["H2SeO3"]
    CultureMech_002796 --> ing19_H2SeO3
    CHEBI_26642["CHEBI:26642"]
    ing19_H2SeO3 -.-> CHEBI_26642
    ing20_H3BO3["H3BO3"]
    CultureMech_002796 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002796 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
2.9703 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.336634 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.415842 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.049505 G_PER_L CHEBI:32588
potassium chloride
5 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.247525 G_PER_L CHEBI:31206
ammonium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0693069 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.990099 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Sulfur
S 32.067 g/mol
2.9703 G_PER_L CHEBI:26833
sulfur atom
10 NaHCO3
CHO3.Na 84.006 g/mol
0.990099 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.15 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
16 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.04 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
17 Na2WO2 x 2 H2O 0.03 G_PER_L
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
0.02 G_PER_L CHEBI:53001
nickel sulfate
20 H2SeO3
H2O3Se 128.973 g/mol
0.01 G_PER_L CHEBI:26642
selenous acid
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients except NaHCO3 and sulfur, adjust pH to 7.5 with NaOH and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.06 g sulfur) under a stream of CO2-O2 (93:7, v/v) gas mixture, seal with butyl rubber stoppers and readjust pH to 7.5 with NaOH. Pressurize the inoculated vessel to 100 kPa CO2-O2 (93:7, v/v).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.635509Z mediadive-import Imported from MediaDive Source: JCM, ID: J446
2026-03-13T06:19:15.768637Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002796
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.207142+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MJTSO MEDIUM" to "mjtso_medium" for consistent matching
2026-06-05T07:41:35.107181+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.184807+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.