methanobacterium_adherens_medium

CultureMech:002797 archaea COMPLEX LIQUID
Original name: METHANOBACTERIUM ADHERENS MEDIUM

Overview

External ontology
mediadive.medium:J447 (source link unavailable) — METHANOBACTERIUM ADHERENS MEDIUM
Applications
  • Microbial cultivation
pH
7.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=447

Composition

flowchart LR
    CultureMech_002797["methanobacterium_adherens_medium"]
    ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002797 --> ing0_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002797 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002797 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002797 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_002797 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_002797 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_002797 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002797 --> ing7_Yeast_extract
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_002797 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002797 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Resazurin["Resazurin"]
    CultureMech_002797 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002797 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002797 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002797 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_H3BO3["H3BO3"]
    CultureMech_002797 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002797 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002797 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002797 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002797 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002797 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NaOH["NaOH"]
    CultureMech_002797 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002797 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002797 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_Biotin["Biotin"]
    CultureMech_002797 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_002797 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002797 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002797 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Riboflavin["Riboflavin"]
    CultureMech_002797 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002797 --> ing28_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing28_Nicotinic_acid -.-> CHEBI_15940
    ing29_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002797 --> ing29_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_Calcium_pantothenate -.-> CHEBI_31345
    CultureMech_002797 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.148221 G_PER_L CHEBI:86158
calcium chloride dihydrate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.96443 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.247036 G_PER_L CHEBI:31206
ammonium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
0.197628 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 KCl
Cl.K 74.551 g/mol
0.494071 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
19.7628 G_PER_L CHEBI:26710
sodium chloride
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.0810277 G_PER_L CHEBI:32954
sodium acetate
8 Yeast extract 0.988142 G_PER_L
9 Vitamin B12 0.0001494071 G_PER_L CHEBI:176843
vitamin B12
 [Merged 2 duplicates: 4.94071e-05, 0.0001]
10 NaHCO3
CHO3.Na 84.006 g/mol
2.47036 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Resazurin
C12H7NO4 229.191 g/mol
0.000988142 G_PER_L CHEBI:8806
Resazurin
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.494071 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.42 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.17 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.21 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
29 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
30 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
32 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients except NaHCO3 and Na2S x 9H2O, and bring to a boil for 5 min, then, cool down under a stream of a H2-CO2 (80:20, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Filter-sterilize NaHCO3 as an 8% solution while gassing with N2-CO2 (80:20, v/v). Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution to the medium and readjust pH to 7.6 - 7.8 with a sterile 1% Na2CO3 solution (stocked anaerobically). Prior to inoculation, add the sterile Na2S x 9H2O solution. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
  2. Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.639434Z mediadive-import Imported from MediaDive Source: JCM, ID: J447
2026-03-13T03:08:56.476481Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:15.782073Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002797
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.222040+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOBACTERIUM ADHERENS MEDIUM" to "methanobacterium_adherens_medium" for consistent matching
2026-06-05T07:41:30.604257+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.619591+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.