anoxic_medium_for_strain_acbe2_1

CultureMech:002801 bacterial DEFINED LIQUID
Original name: ANOXIC MEDIUM FOR STRAIN AcBE2-1

Overview

External ontology
mediadive.medium:J451 (source link unavailable) — ANOXIC MEDIUM FOR STRAIN AcBE2-1
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=451

Composition

flowchart LR
    CultureMech_002801["anoxic_medium_for_strain_acbe2_1"]
    ing0_NaCl["NaCl"]
    CultureMech_002801 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002801 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002801 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_002801 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002801 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002801 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Na2SO4["Na2SO4"]
    CultureMech_002801 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_NaNO3["NaNO3"]
    CultureMech_002801 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_HCl["HCl"]
    CultureMech_002801 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002801 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002801 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002801 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002801 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002801 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002801 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002801 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002801 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NaOH["NaOH"]
    CultureMech_002801 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002801 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002801 --> ing19_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_002801 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_D_Biotin["D-Biotin"]
    CultureMech_002801 --> ing21_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_D_Biotin -.-> CHEBI_15956
    ing22_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_002801 --> ing22_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_D_Calcium_pantothenate -.-> CHEBI_31345
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002801 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002801 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002801 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002801 --> ing26_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.968992 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.387597 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.193798 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 KCl
Cl.K 74.551 g/mol
0.484496 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.242248 G_PER_L CHEBI:31206
ammonium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.145349 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Na2SO4
2Na.O4S 142.043 g/mol
0.0678295 G_PER_L CHEBI:32149
sodium sulfate
8 NaNO3
NO3.Na 84.994 g/mol
0.411822 G_PER_L CHEBI:63005
sodium nitrate
9 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
19 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
20 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
21 Vitamin B12 0.04 G_PER_L CHEBI:176843
vitamin B12
22 D-Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
23 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
25 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.1 G_PER_L CHEBI:30753
4-aminobenzoic acid
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
27 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride

Preparation

  1. Use sodium acetate instead of 17 beta-estradiol donor. The former can be added prior to autoclaving. After autoclaving and cooling under N2-CO2 (80:20, v/v), add the following components to the medium:
  2. The final pH of the medium should be about 7.2. All culture vessels with estradiol as substrate are incubated at 28C in the dark for 8 - 10 days. The culture with estradiol should be briefly shaken once a day.
  3. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Solutions

84 g/L NaHCO3 solution

Dose: 30 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.650286Z mediadive-import Imported from MediaDive Source: JCM, ID: J451
2026-01-28T19:48:20.037016Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:33.253298+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:15.815429Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002801
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.259730+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ANOXIC MEDIUM FOR STRAIN AcBE2-1" to "anoxic_medium_for_strain_acbe2_1" for consistent matching
2026-05-16T09:03:24.571409+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:49.266655+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.370046+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.