1_5_nutrient_agar_no_2

CultureMech:002803 bacterial COMPLEX SOLID_AGAR
Original name: 1/5 NUTRIENT AGAR NO. 2

Overview

External ontology
mediadive.medium:J453 (source link unavailable) — 1/5 NUTRIENT AGAR NO. 2
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=453

Composition

flowchart LR
    CultureMech_002803["1_5_nutrient_agar_no_2"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_002803 --> ing0_Bacto_peptone
    ing1_Beef_extract["Beef extract"]
    CultureMech_002803 --> ing1_Beef_extract
    ing2_NaCl["NaCl"]
    CultureMech_002803 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_002803 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 2 G_PER_L
2 Beef extract 2 G_PER_L
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
4 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.0 - 7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.656538Z mediadive-import Imported from MediaDive Source: JCM, ID: J453
2026-03-13T06:19:15.825340Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002803
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.272133+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 NUTRIENT AGAR NO. 2" to "1_5_nutrient_agar_no_2" for consistent matching
2026-06-05T07:40:15.647581+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.