spirochaeta_californiensis_medium

CultureMech:002804 bacterial COMPLEX LIQUID
Original name: SPIROCHAETA CALIFORNIENSIS MEDIUM

Overview

External ontology
mediadive.medium:J454 (source link unavailable) — SPIROCHAETA CALIFORNIENSIS MEDIUM
Applications
  • Microbial cultivation
pH
9.2
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=454

Composition

flowchart LR
    CultureMech_002804["spirochaeta_californiensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002804 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2CO3["Na2CO3"]
    CultureMech_002804 --> ing1_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing1_Na2CO3 -.-> CHEBI_29377
    ing2_NaHCO3["NaHCO3"]
    CultureMech_002804 --> ing2_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing2_NaHCO3 -.-> CHEBI_32139
    ing3_KCl["KCl"]
    CultureMech_002804 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002804 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002804 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_NH4Cl["NH4Cl"]
    CultureMech_002804 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002804 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002804 --> ing8_Yeast_extract
    ing9_Glucose["Glucose"]
    CultureMech_002804 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_Resazurin["Resazurin"]
    CultureMech_002804 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Biotin["Biotin"]
    CultureMech_002804 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_002804 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002804 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002804 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_002804 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002804 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002804 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_002804 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002804 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Lipoic_acid["Lipoic acid"]
    CultureMech_002804 --> ing20_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20_Lipoic_acid -.-> CHEBI_16494
    ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002804 --> ing21_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing22_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002804 --> ing22_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing22_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing23_Na2SeO3["Na2SeO3"]
    CultureMech_002804 --> ing23_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing23_Na2SeO3 -.-> CHEBI_48843
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002804 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002804 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing26_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002804 --> ing26_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing26_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002804 --> ing27_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002804 --> ing28_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing29_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002804 --> ing29_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing29_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
10000 G_PER_L CHEBI:26710
sodium chloride
2 Na2CO3
CO3.2Na 105.988 g/mol
920 G_PER_L CHEBI:29377
sodium carbonate
3 NaHCO3
CHO3.Na 84.006 g/mol
8000 G_PER_L CHEBI:32139
sodium hydrogencarbonate
4 KCl
Cl.K 74.551 g/mol
66.6667 G_PER_L CHEBI:32588
potassium chloride
5 K2HPO4
HO4P.2K 174.174 g/mol
66.6667 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
33.3333 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 NH4Cl
Cl.H4N 53.492 g/mol
333.333 G_PER_L CHEBI:31206
ammonium chloride
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
133.333 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 Yeast extract 166.667 G_PER_L
10 Glucose
C6H12O6 180.156 g/mol
1666.67 G_PER_L CHEBI:17234
glucose
11 Resazurin
C12H7NO4 229.191 g/mol
0.333333 G_PER_L CHEBI:8806
Resazurin
12 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
13 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
16 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
17 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
19 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
22 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
23 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
24 Na2SeO3
2Na.O3Se 172.937 g/mol
0.1 G_PER_L CHEBI:48843
disodium selenite
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
27 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.1 G_PER_L CHEBI:86360
manganese(II) sulfate
28 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
29 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
30 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate

Preparation

  1. Dissolve components except Na2CO3, NaHCO3 and Na2S x 9H2O in 700 ml distilled water and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. Separately dissolve Na2CO3 and NaHCO3 in 300 ml distilled water, filter-sterilize, and add to the medium aseptically and anaerobically. Adjust pH to 9.0 - 9.5, if necessary. Prior to inoculation, reduce the medium by the addition of a 5% Na2S x 9H2O solution autoclaved under a N2 atmosphere.
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals.
  3. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.658038Z mediadive-import Imported from MediaDive Source: JCM, ID: J454
2026-01-28T19:48:33.641886Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.835616Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002804
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.283863+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPIROCHAETA CALIFORNIENSIS MEDIUM" to "spirochaeta_californiensis_medium" for consistent matching
2026-06-05T07:41:46.266336+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.612061+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.