Overview
- External ontology
- mediadive.medium:J460 (source link unavailable) — FLUID THIOGLYCOLLATE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=460
Composition
flowchart LR
CultureMech_002809["fluid_thioglycollate_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002809 --> ing0_Trypticase_peptone
ing1_L_Cystine["L-Cystine"]
CultureMech_002809 --> ing1_L_Cystine
CHEBI_16283["CHEBI:16283"]
ing1_L_Cystine -.-> CHEBI_16283
ing2_Glucose["Glucose"]
CultureMech_002809 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Yeast_extract["Yeast extract"]
CultureMech_002809 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_002809 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Sodium_thioglycollate["Sodium thioglycollate"]
CultureMech_002809 --> ing5_Sodium_thioglycollate
CHEBI_86481["CHEBI:86481"]
ing5_Sodium_thioglycollate -.-> CHEBI_86481
ing6_Resazurin["Resazurin"]
CultureMech_002809 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Agar["Agar"]
CultureMech_002809 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 15 G_PER_L | ||
| 2 |
L-Cystine
C6H12N2O4S2 240.306 g/mol
|
0.5 G_PER_L |
CHEBI:16283
L-cystine
|
|
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Yeast extract | 5 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Sodium thioglycollate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust pH to 7.1 with Na2CO3 (10%, w/v), if necessary. Prepare medium under a N2 atmosphere.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.670815Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J460 |
| 2026-03-13T06:19:15.855766Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002809 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.308346+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FLUID THIOGLYCOLLATE MEDIUM" to "fluid_thioglycollate_medium" for consistent matching |
| 2026-06-05T07:41:00.478980+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |